Document 0705
 DOCN  M9460705
 TI    [Cryptococcus neoformans chemotyping. Review of the literature. New
       epidemiologic data on cryptococcosis. Our experience with the use of
       C.G.B. media in the study of this yeast]
 DT    9404
 AU    de Melo NT; Lacaz C da S; Charbel CE; Pereira AD; Heins-Vaccari EM;
       Franca-Netto AS; Machado L dos R; Livramento JA; Instituto de Medicina
       Tropical de Sao Paulo (Laboratorio de; Micologia Medica), Brasil.
 SO    Rev Inst Med Trop Sao Paulo. 1993 Sep-Oct;35(5):469-78. Unique
       Identifier : AIDSLINE MED/94160083
 AB    The purpose of this work was to collect the main information from the
       literature about the biotyping of Cryptococcus neoformans. The more
       up-to date research concerning the epidemiology of cryptococcosis
       comprising quite a few articles, mainly after the advent of AIDS, was
       also reviewed. The Cryptococcus neoformans varieties neoformans and
       gattii are well defined biochemically nowadays chiefly through the
       C.G.B. medium, according to Kwon-Chung et al. (1982). The isolation of
       C. neoformans var. gattii from flowers and leaves of Eucalyptus
       camaldulensis and Eucalyptus tereticornis, specially in Australia,
       through the works of Ellis & Pfeiffer (1990) and Pfeiffer & Ellis (1992)
       permitted very interesting epidemiological investigations on C.
       neoformans, a capsulated yeast by which Sanfelice, in Italy (1894; 1895)
       attracted attention of medical class. Busse, in 1894, described the
       first human case of cryptococcosis under the presentation of a bone
       lesion simulating sarcoma. In this paper, the Brazilian researchers
       focused on this subject were pointed out, followed by the Author's
       experience with the C.G.B. medium (L-canavanine, glycine and bromothymol
       blue) proposed by Kwon-Chung et al. (1982) with very good results. It
       was possible with such medium the study of 50 C.N.S. liquor samples,
       being 39 from AIDS patients (78%) and 11 from non-AIDS ones (22%).
       Thirty-seven out of the 39 HIV-positive patients (74%) were identified
       as C. neoformans var. gattii. From the negative HIV, 8 (16%) were
       classified as C. neoformans var. neoformans and 3 (6%) as C. neoformans
       var. gattii. We could not perform the serotyping of the above referred
       samples. It is evident anyway that in Brazil there exist both varieties
       gattii and neoformans, agents of neurocryptococcosis, including AIDS
       patients. The importance of neurocryptococcosis, mainly among AIDS
       patients, is stressed here, showing once more the value of C.G.B. medium
       in the typing of C. neoformans in its two varieties. Also, it is of
       relevant importance the demonstration that some species of eucalyptus
       may act as host-trees of C. neoformans var. gattii.
 DE    Animal  Bromthymol Blue  Canavanine
       Cryptococcosis/*EPIDEMIOLOGY/PHYSIOPATHOLOGY  Cryptococcus
       neoformans/*CLASSIFICATION/ISOLATION & PURIF  Culture Media  English
       Abstract  Glycine  Serotyping  JOURNAL ARTICLE  REVIEW  REVIEW, TUTORIAL

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