Document 0795
 DOCN  M9460795
 TI    Morphological differentiation of human SH-SY5Y neuroblastoma cells
       inhibits human immunodeficiency virus type 1 infection.
 DT    9404
 AU    Vesanen M; Salminen M; Wessman M; Lankinen H; Sistonen P; Vaheri A;
       Department of Virology, University of Helsinki, Finland.
 SO    J Gen Virol. 1994 Jan;75 ( Pt 1):201-6. Unique Identifier : AIDSLINE
       MED/94157463
 AB    We have studied human immunodeficiency virus type 1 (HIV-1) infection in
       human SH-SY5Y neuroblastoma cells at various stages of morphological
       differentiation. Two days' treatment of the cells with retinoic acid
       (RA) or dibutyryl cAMP (db-cAMP) resulted in the appearance of elongated
       neurites and enhanced production of 160K to 200K neurofilament proteins
       as shown by indirect immunofluorescence. DNA synthesis was reduced only
       in RA-treated cells as detected by 5-bromo-2'-deoxyuridine
       incorporation. The cells were infected with two T-lymphotropic virus
       strains (IIIB and NDK) and two fresh isolates (39001 and 46001) from
       bronchoalveolar lavage samples of AIDS patients. The latter two isolates
       were unable to form syncytia in infected CD4-positive T-lymphoblastoid
       C8166 cells which was in contrast to our T-lymphotropic virus strains.
       Interphase in situ hybridization showed that 14 to 16% of SH-SY5Y cells
       become positive for HIV-1 DNA. Regardless of the virus strain,
       morphological differentiation of the cells with RA or db-cAMP inhibited
       infection by 50% at a single cell in situ resolution. Nested PCR
       confirmed the presence of proviral DNA in the infected cells. These
       results show that human neuroblastoma cells, tumour cells of
       neuroectodermal origin, can be infected by different HIV-1 isolates and
       that the infection is inhibited by neurotypic cell differentiation.
 DE    Base Sequence  Bucladesine/PHARMACOLOGY  Cell Differentiation/DRUG
       EFFECTS/PHYSIOLOGY  Human  HIV-1/*PHYSIOLOGY  Molecular Sequence Data
       Nerve Growth Factors/PHARMACOLOGY  Neuroblastoma
       Neurons/CYTOLOGY/*MICROBIOLOGY  Support, Non-U.S. Gov't
       Tretinoin/PHARMACOLOGY  Tumor Cells, Cultured  JOURNAL ARTICLE

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