Document 0808
 DOCN  M9460808
 TI    Cytochemical analysis of human T cell leukaemia virus I LTR-regulated
       beta-galactosidase gene expression using a novel integrated cell system.
 DT    9404
 AU    Copeland KF; Haaksma AG; Derse D; Goudsmit J; Heeney JL; Department of
       Chronic and Infectious Diseases. TNO-MBL, Rijswijk,; The Netherlands.
 SO    J Virol Methods. 1993 Dec 15;45(2):161-7. Unique Identifier : AIDSLINE
       MED/94157016
 AB    To develop a reporter system to study the response of an integrated
       retroviral LTR and cellular and viral events which influence
       transcription, the 5' LTR of HTLV-1 was coupled to the Escherichia coli
       beta-galactosidase gene (lacZ). This construct was assembled within a
       vector containing the neomycin resistance gene controlled by the SV40
       promoter, and introduced into HeLa cells. Expression from the LTR in one
       clone was upregulated by positive regulators of HTLV-1 expression,
       including 12-O-tetradecanoylphorbol-13-acetate (TPA) and the HTLV-1
       transregulatory protein (tax), as has been previously reported using
       transient transfection assays. This method proved to be a rapid and
       reproducible assay for the measurement of integrated viral LTR
       activation in a single cell system.
 DE    beta-Galactosidase/*GENETICS  Escherichia coli/GENETICS  Evaluation
       Studies  Gene Expression Regulation, Viral/DRUG EFFECTS  Gene Products,
       tax/PHARMACOLOGY  Genes, Reporter  Genes, Viral  Genetic Vectors  Hela
       Cells  Histocytochemistry  Human  HTLV-I/*ENZYMOLOGY/*GENETICS  Lac
       Operon  *Repetitive Sequences, Nucleic Acid  Support, Non-U.S. Gov't
       Tetradecanoylphorbol Acetate/PHARMACOLOGY  Virology/METHODS  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).