Document 0815
 DOCN  M9460815
 TI    Cytotoxicity of a shiga toxin A subunit-CD4 fusion protein to human
       immunodeficiency virus-infected cells.
 DT    9404
 AU    al-Jaufy AY; Haddad JE; King SR; McPhee RA; Jackson MP; Department of
       Immunology and Microbiology, Wayne State University; School of Medicine,
       Detroit, Michigan 48201.
 SO    Infect Immun. 1994 Mar;62(3):956-60. Unique Identifier : AIDSLINE
       MED/94156493
 AB    Shiga toxin (STX) is a ribosome-inactivating cytotoxin produced by
       Shigella dysenteriae serotype 1. The enzymatic domain of the STX A
       polypeptide has been defined by introducing amino- and carboxy-terminal
       deletions in the polypeptide and assessing activity in a cell-free
       translation system. Three recombinant forms of StxA which possess
       enzymatic activity were genetically fused to a 165-amino-acid
       polypeptide derived from CD4, the cellular receptor for human
       immunodeficiency virus type 1 (HIV-1). This strategy eliminated the STX
       receptor-binding subunit and directed the hybrid toxins to cells
       expressing the HIV-1 surface glycoprotein gp120. A bacterial lysate
       containing these toxin chimeras killed the HIV-1-infected T-cell line
       8E5 but was not cytotoxic toward the uninfected parental cell line
       A3.01. This cytotoxic activity was specifically inhibited by monoclonal
       antibodies which block the interaction between CD4 and gp120. These
       StxA-CD4 hybrids add to the repertoire of recombinant fusion proteins
       which possess the capacity to selectively kill HIV-1-infected T cells.
 DE    Antigens, CD4/*PHYSIOLOGY  Bacterial Toxins/*TOXICITY  Cell Line
       Cytotoxins/*TOXICITY  Human  HIV Envelope Protein gp120/PHYSIOLOGY
       HIV-1/*PHYSIOLOGY  Recombinant Fusion Proteins/*TOXICITY  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).