Document 0865
 DOCN  M9460865
 TI    Two- and three-dimensional HCN experiments for correlating base and
       sugar resonances in 15N,13C-labeled RNA oligonucleotides.
 DT    9404
 AU    Sklenar V; Peterson RD; Rejante MR; Feigon J; Department of Chemistry
       and Biochemistry, University of; California, Los Angeles 90024.
 SO    J Biomol NMR. 1993 Nov;3(6):721-7. Unique Identifier : AIDSLINE
       MED/94154514
 AB    New 2D and 3D 1H-13C-15N triple resonance experiments are presented
       which allow unambiguous assignments of intranucleotide H1'-H8(H6)
       connectivities in 13C- and 15N-labeled RNA oligonucleotides. Two
       slightly different experiments employing double INEPT forward and back
       coherence transfers are optimized to obtain the H1'-C1'-N9/N1 and
       H8/H6-C8/C6-N9/N1 connectivities, respectively. The correlation of H1'
       protons to glycosidic nitrogens N9/N1 is obtained in a nonselective
       fashion. To correlate H8/H6 with their respective glycosidic nitrogens,
       selective 13C-refocusing and 15N-inversion pulses are applied to
       optimize the magnetization transfers along the desired pathway. The
       approach employs the heteronuclear one-bond spin-spin interactions and
       allows the 2D 1H-15N and 3D 1H-13C-15N chemical shift correlation of
       nuclei along and adjacent to the glycosidic bond. Since the
       intranucleotide correlations obtained are based exclusively on
       through-bond scalar interactions, these experiments resolve the
       ambiguity of intra- and internucleotide H1'-H8(H6) assignments obtained
       from the 2D NOESY spectra. These experiments are applied to a 30-base
       RNA oligonucleotide which contains the binding site for Rev protein from
       HIV.
 DE    Base Sequence  Carbohydrates/*CHEMISTRY  Carbon Isotopes  Hydrogen
       Molecular Sequence Data  Nitrogen Isotopes  *Nuclear Magnetic Resonance
       Nucleosides/*CHEMISTRY  Oligoribonucleotides/*CHEMISTRY  Protons
       RNA/*CHEMISTRY  Support, U.S. Gov't, Non-P.H.S.  Support, U.S. Gov't,
       P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).