Document 0876
 DOCN  M9460876
 TI    IgMs produced by two acquired immune deficiency syndrome lymphoma cell
       lines: Ig binding specificity and VH-gene putative somatic mutation
       analysis.
 DT    9404
 AU    Ng VL; Hurt MH; Fein CL; Khayam-Bashi F; Marsh J; Nunes WM; McPhaul LW;
       Feigal E; Nelson P; Herndier BG; et al; Department of Laboratory
       Medicine, School of Medicine, University; of California, San Francisco.
 SO    Blood. 1994 Feb 15;83(4):1067-78. Unique Identifier : AIDSLINE
       MED/94154268
 AB    Two B-cell lines, 2F7 and 10C9, were established by single cell cloning
       from biopsies obtained from two acquired immune deficiency syndrome
       patients with Burkitt's lymphoma. Representation of the original tumors
       was verified by demonstration of (1) identical biallelic rearrangement
       of Ig genes for 2F7 and (2) shared idiotype for 10C9. Both cell lines
       displayed cell-surface Ig and secreted Ig (IgM lambda for 2F7, IgM kappa
       for 10C9). IgMs from both cell lines immunoprecipitated actin; in
       addition, 2F7 IgM lambda immunoprecipitated recombinant human
       immunodeficiency virus type 1 (HIV-1) gp 160. 2F7 IgM lambda did not
       react with other autoantigens (double-stranded and single-stranded DNA,
       actin, bovine serum albumin, IgG), whereas 10C9 IgM kappa reacted with
       human IgG. The 2F7 IgM heavy-chain variable region (VH) showed a 95%
       nucleotide homology with a previously sequenced VHIII germline gene,
       hv3019b9, whereas the 10C9 IgM VH showed a 95% homology with a
       previously sequenced VHIV germline gene, VH4.21. Use of minimally
       modified VH genes and demonstration of reactivity with chronically
       present antigens (ie, actin, HIV-1 gp 160, or human IgG) suggests that B
       cells in HIV-1-infected individuals proliferating in response to chronic
       antigenic stimulation may be at increased risk for lymphomagenesis.
 DE    Amino Acid Sequence  Antibodies, Anti-Idiotypic/IMMUNOLOGY  Antibody
       Specificity  Autoantigens/ANALYSIS  Base Sequence  Blotting, Southern
       Cell Line  Cell Line, Transformed  *DNA Mutational Analysis  DNA Primers
       DNA, Neoplasm/ANALYSIS/GENETICS  Gene Rearrangement  *Genes,
       Immunoglobulin  Human  HIV-1  IgM/BIOSYNTHESIS/*GENETICS  Immunoenzyme
       Techniques  Immunoglobulin Variable Region/BIOSYNTHESIS/*GENETICS
       Immunoglobulins, Heavy-Chain/BIOSYNTHESIS/*GENETICS  Lymphoma,
       AIDS-Related/*GENETICS/*IMMUNOLOGY  Molecular Sequence Data  Polymerase
       Chain Reaction  Signal Peptides/GENETICS  Support, Non-U.S. Gov't
       Support, U.S. Gov't, P.H.S.  Tumor Cells, Cultured  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).