Document 0971
 DOCN  M9460971
 TI    Identification and characterization of novel antigenic determinants and
       endogenous retroviruses in normal and neoplastic canine lymphoid cells.
 DT    9406
 AU    Jure MN; No affiliation given
 SO    Diss Abstr Int [B]; 54(2):685 1993. Unique Identifier : AIDSLINE
       ICDB/94696659
 AB    Studies were conducted to characterize the canine species as a model of
       lymphomagenesis. The first objective was to develop monoclonal
       antibodies against canine lymphocyte surface antigens and apply these
       reagents in the characterization of canine lymphoid malignancies.
       Second, since several lines of evidence indicated that endogenous
       retroviruses were expressed in lymphoid neoplasms and transformed cells,
       a study designed to identify endogenous retroviruses in the canine
       genome was undertaken. Several monoclonal antibodies were produced.
       Monoclonal antibody 2E9 recognized a panleukocyte antigen absent from
       certain T-cell hyperplasias that appears as a useful reagent for the
       discrimination between this type of hyperplasia and lymphomas.
       Monoclonal antibody 8E3 recognized an antigen detected on the surface of
       lymphoid malignancies, mitogen-stimulated lymphocytes, and transformed
       cell lines but not on normal resting cells. Monoclonal antibody 7A4
       bound to an activation antigen also expressed on immature lymphocytes.
       Monoclonal antibodies 7A4 and 8E3 appear as useful reagents for the
       classification, diagnosis and prognosis of canine lymphoid neoplasms.
       Reverse transcriptase (RT) activity (Mn++ dependent) was detected in the
       supernatant of a canine transformed cell line. Particles containing RT
       activity banded in sucrose at a density of 1.15 g/cc. RNA contained in
       these particles was subjected to a RT-PCR-based procedure using
       degenerate primers derived from a conserved region of the retroviral
       polymerase gene. A predicted product of 136 bp was obtained, cloned and
       sequenced. The amplified product showed 61% homology to the simian type
       D retrovirus SRV-2 and complete homology with a consensus amino acid
       sequence derived from known retroviruses. A probe derived from the
       cloned amplified fragment was used to hybridize canine genomic DNA.
       Southern blot analysis showed that between 1 and 10 copies of this gene
       are present in the canine genome. In addition, a canine genomic library
       was screened with the amplified product. Several different recombinant
       phages were purified and partially characterized. Restriction enzyme
       analysis showed that conserved restriction sites in the pol gene were
       found in the different clones. (Full text available from University
       Microfilms International, Ann Arbor, MI, as Order No. AAD93-18789)
 DE    Amino Acid Sequence  Animal  Antibodies, Monoclonal/DIAGNOSTIC USE
       *Antigenic Determinants  Antigens, Neoplasm/*ANALYSIS  Cell
       Transformation, Neoplastic/GENETICS/IMMUNOLOGY  Dogs  Gene Amplification
       Lymphocytes/DRUG EFFECTS/IMMUNOLOGY  Lymphoma/*CHEMISTRY/DIAGNOSIS
       Mitogens/PHARMACOLOGY  Polymerase Chain Reaction
       Retroviridae/GENETICS/*ISOLATION & PURIF  Retroviruses Type D,
       Simian/*GENETICS/IMMUNOLOGY  Sequence Homology, Amino Acid  THESIS

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).