Document 0001
 DOCN  M9460001
 TI    Sequence-specific recognition of the HIV-1 long terminal repeat by
       distamycin: a DNAase I footprinting study.
 DT    9408
 AU    Feriotto G; Mischiati C; Gambari R; Biochemistry Institute, Ferrara
       University, Italy.
 SO    Biochem J. 1994 Apr 15;299 ( Pt 2):451-8. Unique Identifier : AIDSLINE
       MED/94226609
 AB    Pharmacological modulation of the interaction between transcription
       factors and target DNA sequences of cellular and viral genes could have
       important effects in the experimental therapy of a large variety of
       human pathologies. For instance, alteration of the DNA/protein
       interaction might be among the molecular mechanisms of action of
       DNA-binding drugs, leading to an inhibition of the expression of genes
       involved in the control of in vitro and in vivo growth of neoplastic
       cells and virus DNA replication. Natural oligopeptides, such as
       distamycin, are powerful inhibitors of the interaction between nuclear
       factors and target DNA sequences and, therefore, have been proposed as
       compounds retaining antibiotic, antineoplastic and antiviral properties.
       In this study we performed DNAase I footprinting analysis using a PCR
       product mimicking a region of the long terminal repeat (LTR) of the
       human immunodeficiency type 1 (HIV-1) retrovirus. The data obtained
       suggest that distamycin binds to different regions of the HIV-1 LTR
       depending on the DNA sequence. Electrophoretic mobility shift assays
       using both crude nuclear extracts from the Jurkat T-lymphoid cell line
       and the recombinant proteins transcription factor IID and Sp1 suggest
       that distamycin differentially inhibits the interaction of these two
       proteins with their specific DNA target sequences, in good agreement
       with the results obtained by DNAase I footprinting analysis.
 DE    Base Sequence  Binding Sites  Consensus Sequence  Deoxyribonuclease I
       Distamycins/*METABOLISM  DNA, Viral/*METABOLISM  Genes, Viral  Genome,
       Viral  Human  *HIV Long Terminal Repeat  HIV-1/*GENETICS/METABOLISM
       Molecular Sequence Data  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).