Document 0030
 DOCN  M9460030
 TI    Nucleotide sequence analysis of IS1533 from Leptospira borgpetersenii:
       identification and expression of two IS-encoded proteins.
 DT    9408
 AU    Zuerner RL; Leptospirosis and Mycobacteriosis Research Unit, U.S.
       Department; of Agriculture, National Animal Disease Center, Ames, Iowa
       50010.
 SO    Plasmid. 1994 Jan;31(1):1-11. Unique Identifier : AIDSLINE
       GENBANK/M82880
 AB    The nucleotide sequence of IS1533, an insertion sequence-like element
       cloned from the spirochete Leptospira borgpetersenii, was determined.
       IS1533 contains imperfect terminal inverted repeats (IVR) of 31 bp
       flanking a 1402-bp internal sequence. A putative target sequence was
       identified, and insertion may result in duplication of 2 bp. The
       internal sequence has a single open reading frame (ORF). IS1533 encodes
       two proteins (43.5 and 41 kDa) initiating alternatively at either the
       first or the second AUG codons of the ORF. These proteins are related to
       a recently recognized family of IS-encoded transposases and bacterial
       recombinases, all which share a region of homology with the active site
       of the HIV reverse transcriptase. The IS1533-encoded proteins were
       expressed in Escherichia coli. Both the 43.5- and 41-kDa proteins bound
       IS1533 DNA probes in a Southwestern blot assay. These data suggest that
       one or both proteins function during transposition of IS1533.
 DE    Amino Acid Sequence  Bacterial Proteins/BIOSYNTHESIS/*GENETICS  Base
       Sequence  Cloning, Molecular  Comparative Study  *DNA Insertion Elements
       Introns  Leptospira/*GENETICS/METABOLISM  Molecular Sequence Data
       Mutagenesis, Insertional  Oligodeoxyribonucleotides  Open Reading Frames
       *Plasmids  Repetitive Sequences, Nucleic Acid  Restriction Mapping  RNA,
       Messenger/BIOSYNTHESIS/METABOLISM  Sequence Homology, Amino Acid
       Sequence Homology, Nucleic Acid  Terminator Regions (Genetics)
       Transcription, Genetic  Translation, Genetic  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).