Document 0037
 DOCN  M9460037
 TI    The nature of the autoimmune antibody repertoire in human
       immunodeficiency virus type 1 infection.
 DT    9408
 AU    Ditzel HJ; Barbas SM; Barbas CF 3rd; Burton DR; Department of
       Immunology, Scripps Research Institute, La Jolla,; CA 92037.
 SO    Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3710-4. Unique Identifier :
       AIDSLINE GENBANK/U07196
 AB    Human immunodeficiency virus type 1 (HIV-1) seropositive donors
       typically have high serum antibody titers to a range of autoantigens,
       and the corresponding autoantibodies have been suggested to be of
       importance in the pathogenesis of HIV-1 infection. We have prepared 38
       IgG human monoclonal autoantibodies from asymptomatic HIV-1 seropositive
       donors with elevated serum titers to autoantigens by construction of Fab
       combinatorial libraries on the surface of phage and affinity selection
       using a range of autoantigens, including double-stranded DNA, major
       histocompatibility complex class II, CD14, epidermal growth factor
       receptor, and ganglioside GD2. The autoantibodies are shown to be of
       moderate affinity and exhibit marked cross-reactivity with a range of
       antigens. This contrasts with the specific high-affinity antibodies
       selected (i) against infectious agents using the same libraries and (ii)
       against one of the autoantigens using a library from a donor with
       established autoimmune disease. The results lend no support to the
       presence of specific autoantibodies in HIV-1 infection and instead
       suggest attention should be focused on the pathological significance of
       high serum levels of antibodies capable of interacting with multiple
       molecular species.
 DE    Amino Acid Sequence  Antibody Specificity
       Autoantibodies/GENETICS/*IMMUNOLOGY  Autoantigens/*IMMUNOLOGY  Base
       Sequence  Comparative Study  DNA Primers/CHEMISTRY  Gene Library  Genes,
       Immunoglobulin  Human  HIV Infections/*IMMUNOLOGY  Immunoglobulin
       Variable Region/GENETICS  Immunoglobulins, Fab/METABOLISM
       Immunoglobulins, Heavy-Chain/GENETICS  Male  Molecular Sequence Data
       Sequence Alignment  Sequence Homology, Amino Acid  Support, Non-U.S.
       Gov't  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).