Document 0083
 DOCN  M9460083
 TI    Experimental human infection with Haemophilus ducreyi.
 DT    9408
 AU    Spinola SM; Wild LM; Apicella MA; Gaspari AA; Campagnari AA; Department
       of Medicine, Indiana University, Indianapolis.
 SO    J Infect Dis. 1994 May;169(5):1146-50. Unique Identifier : AIDSLINE
       MED/94223082
 AB    Four subjects were experimentally infected with Haemophilus ducreyi.
       Lesions developed only at sites where live bacteria were inoculated on
       abraded skin. No subject developed fever, lymphadenopathy, or
       disseminated infection during a 3-day observation period. Two subjects
       who were rechallenged 2 months after initial infection also developed
       lesions. The amount of H. ducreyi recovered from 10 of 12 biopsies that
       were semiquantitatively cultured varied widely. Similar histologic
       features were present in initial and second infections. The epidermis
       contained pustules; the dermis contained an infiltrate of T cells and
       macrophages and reactive endothelial cells. Keratinocytes and T cells
       expressed HLA-DR, consistent with a delayed-type hypersensitivity
       response. The subjects did not mount humoral responses to bacterial
       proteins and to lipooligosaccharides after primary and secondary
       challenges. Thus, human experimental infection with H. ducreyi is well
       tolerated and safe. Recruitment of T cells and macrophages into
       chancroid lesions may partially explain the association between
       chancroid and human immunodeficiency virus transmission.
 DE    Adult  Chancroid/IMMUNOLOGY/*PHYSIOPATHOLOGY  Female  Haemophilus
       ducreyi  Human  Male  Support, Non-U.S. Gov't  Support, U.S. Gov't,
       P.H.S.  T-Lymphocytes/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).