Document 0141
 DOCN  M9460141
 TI    Tax protein of human T-lymphotropic virus type I triggers DNA damage.
 DT    9408
 AU    Saggioro D; Majone F; Forino M; Turchetto L; Leszl A; Chieco-Bianchi L;
       Institute of Oncology, Interuniversity Center for Cancer Research;
       (CIRC), University of Padova, Italy.
 SO    Leuk Lymphoma. 1994 Jan;12(3-4):281-6. Unique Identifier : AIDSLINE
       MED/94220956
 AB    Previous findings indicated that in vitro HTLV-I-infected cells are
       highly susceptible to spontaneous and chemically induced DNA-damage. To
       further study the role of different virus gene products in inducing
       chromosome abnormalities, MOLT-3 cells were transiently transfected with
       a tax expressing plasmid (pTax), and assayed for genetic damage by the
       micronucleus test. We found that pTax-transfected cells not only had a
       statistically higher baseline micronucleus value than non-transfected
       control cells, but also were more susceptible to Mitomycin C
       (MMC)-induced DNA damage. Furthermore, the use of human serum containing
       anti-kinetochore antibodies disclosed that tax enhances the clastogenic
       effect of MMC. No increase in total micronucleus frequency was observed
       when MMC treatment preceded pTax transfection, thus suggesting that the
       micronucleus increase might not be due to the additive effect of tax and
       MMC. These findings indicate that the viral tax protein could play an
       important role in inducing the chromosome damage frequently observed in
       HTLV-I-infected cells.
 DE    Antibodies/PHARMACOLOGY  Cell Line  Chloramphenicol
       Acetyltransferase/BIOSYNTHESIS/METABOLISM  *DNA Damage  Fluorescent
       Antibody Technique  Gene Products, tax/BIOSYNTHESIS/*METABOLISM  *Genes,
       pX  Human  HTLV-I/*GENETICS  Lymphoma, T-Cell  Micronuclei/DRUG
       EFFECTS/PHYSIOLOGY/*ULTRASTRUCTURE  Mitomycin C/TOXICITY  Plasmids
       Support, Non-U.S. Gov't  Transfection  Tumor Cells, Cultured  JOURNAL
       ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).