Document 0163
 DOCN  M9460163
 TI    Chiral discrimination of enantiomeric 2'-deoxythymidine 5'-triphosphate
       by HIV-1 reverse transcriptase and eukaryotic DNA polymerases.
 DT    9408
 AU    Yamaguchi T; Iwanami N; Shudo K; Saneyoshi M; Department of Biological
       Sciences, Nishi-Tokyo University,; Yamanashi, Japan.
 SO    Biochem Biophys Res Commun. 1994 Apr 29;200(2):1023-7. Unique Identifier
       : AIDSLINE MED/94234989
 AB    Inhibitory effects of 2'-deoxy-L-thymidine 5'-triphosphate (L-dTTP), the
       enantiomer of the natural substrate D-dTTP, on the activity of mammalian
       DNA polymerases alpha, beta and gamma, Escherichia coli DNA polymerase I
       and human immunodeficiency virus 1 (HIV-1) reverse transcriptase were
       examined. When poly(rA)n-oligo(dT)12-18 was used as the template-primer,
       L-dTTP showed remarkable inhibitory effect on HIV-1 reverse
       transcriptase in competitive fashion with respect to the substrate dTTP.
       In contrast, L-dTTP did not inhibit DNA polymerases alpha and was
       slightly inhibitory to DNA polymerase beta. These results suggest that
       the nuclear DNA polymerases alpha and beta showed high specificity for
       the substrate with the natural configuration of the sugar moiety,
       D-dTTP, exhibiting little or no ability to recognize L-dTTP, whereas
       HIV-1 reverse transcriptase essentially lacked the ability to
       differentiate the D- and L-sugar moieties.
 DE    Animal  Binding, Competitive  Cattle  DNA Polymerase I/ANTAGONISTS &
       INHIB/METABOLISM  DNA Polymerase II/ANTAGONISTS & INHIB/METABOLISM  DNA
       Polymerases/ANTAGONISTS & INHIB/*METABOLISM  HIV-1/*ENZYMOLOGY  In Vitro
       Kinetics  Oligodeoxyribonucleotides/METABOLISM  Poly A/METABOLISM  Rats
       Reverse Transcriptase/ANTAGONISTS & INHIB/*METABOLISM  Stereoisomers
       Substrate Specificity  Thymine
       Nucleotides/CHEMISTRY/*METABOLISM/PHARMACOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).