Document 0187
 DOCN  M9460187
 TI    Purification of antibody-antigen complexes containing recombinant SIV
       proteins: comparison of antigen and antibody-antigen complexes for
       immune priming.
 DT    9408
 AU    Randall RE; Young D; Hanke T; Szawlowski P; Botting C
 SO    Vaccine. 1994 Mar;12(4):351-8. Unique Identifier : AIDSLINE MED/94233851
 AB    This paper describes a general procedure for the two-step purification
       of recombinant proteins as antibody-antigen complexes in which there is
       no uncomplexed antibody or antigen. In this way, immune complexes
       containing the p17, p27, vpr and vpx proteins of simian immunodeficiency
       virus (SIV) have been purified. Antibody-antigen complexes are more
       immunogenic than antigen when administered either alone or with alum.
       The significance of the work is that this general method could be
       modified for the manufacture of immune complexes for incorporation into
       multivalent vaccines.
 DE    Animal  Antigen-Antibody Complex/*IMMUNOLOGY/*ISOLATION & PURIF
       Antigens, Viral/*IMMUNOLOGY  Blotting, Western  Comparative Study
       Electrophoresis, Polyacrylamide Gel  Mice  Recombinant
       Proteins/IMMUNOLOGY  Support, Non-U.S. Gov't  SIV/*IMMUNOLOGY  Viral
       Proteins/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).