Document 0192
 DOCN  M9460192
 TI    HIV-2 and SIV infection of nonprimate cell lines expressing human CD4:
       restrictions to replication at distinct stages.
 DT    9408
 AU    McKnight A; Clapham PR; Weiss RA; Chester Beatty Laboratories, Institute
       of Cancer Research,; London, United Kingdom.
 SO    Virology. 1994 May 15;201(1):8-18. Unique Identifier : AIDSLINE
       MED/94233779
 AB    Mouse, rat, rabbit, cat, and mink cell lines expressing human CD4
       restrict entry of HIV-1 strains at the cell surface. We now report that
       CD4+ cat and rabbit cell lines are permissive to cell fusion induced by
       several HIV-2 and SIV strains. CD4+ rat and mouse cells are only
       sensitive to HIV-2 and SIV fusion in the presence of active amphotericin
       B. HIV-2 and SIV replicate in CD4+ cat and CD4+ mink cells, but not in
       the CD4+ rabbit, rat, or mouse cells. These latter cell lines poorly
       express a transfected DNA molecular clone of HIV-2 DNA. CD4+ rat cells
       are sensitive to entry by HIV-2 (HTLV-1) pseudotypes and also to HIV-2
       if amphotericin B is present. Early reverse transcription then occurs
       without subsequent viral gene expression, but the fusion-sensitive CD4+
       rabbit cells are not permissive for HIV-2 reverse transcription. Our
       observations demonstrate (i) that the cat and mink cell lines express
       everything required except human CD4 for entry and replication by
       several HIV-2 and SIV strains and (ii) that distinct postpenetration
       blocks to viral replication are evident in rabbit and rodent cell lines.
 DE    Amphotericin B/PHARMACOLOGY  Animal  Antigens,
       CD4/PHARMACOLOGY/*PHYSIOLOGY  Base Sequence  Cats  Cell Fusion/DRUG
       EFFECTS  Cell Line, Transformed  DNA Replication  DNA, Viral/ANALYSIS
       Human  HIV Antigens/ANALYSIS  HIV-2/*PHYSIOLOGY  HTLV-I
       Antigens/PHYSIOLOGY  Mice  Mink  Molecular Sequence Data  Rabbits  Rats
       Recombinant Proteins/PHARMACOLOGY  Species Specificity  Support,
       Non-U.S. Gov't  SIV/*PHYSIOLOGY  Transfection  Virus
       Replication/*PHYSIOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).