Document 0202
 DOCN  M9460202
 TI    Stable biological and antigenic characteristics of HIV-2SBL6669 in
       nonpathogenic infection of macaques.
 DT    9408
 AU    Zhang YJ; Putkonen P; Albert J; Ohman P; Biberfeld G; Fenyo EM;
       Department of Virology, Karolinska Institute, Stockholm, Sweden.
 SO    Virology. 1994 May 1;200(2):583-9. Unique Identifier : AIDSLINE
       MED/94233721
 AB    The purpose of the present study was to investigate if the biological
       and antigenic properties of human immunodeficiency virus type 2 change
       over time in cynomolgus macaques (Macaca fascicularis) experimentally
       infected with HIV-2SBL6669. Sequential virus isolates and serum samples
       were obtained during a 2-year period and studied in autologous
       neutralization assays. All six macaques studied seroconverted shortly
       after infection and remained healthy during the observation period.
       Virus could be isolated from all six animals during the first 100 days
       postinfection. Thereafter four monkeys became virus isolation negative,
       either permanently or transiently (two macaques each), whereas two
       macaques remained virus isolation positive during the entire observation
       period. Sequential reisolates from the macaques invariably replicated in
       HUT-78, U937-2, and Jurkat-tat cell lines, similarly to the HIV-2SBL6669
       inoculum virus. The ability to produce neutralizing antibodies
       correlated with positive virus isolations, hence four macaques produced
       neutralizing antibodies against inoculum virus and sequential
       reisolates. Once the neutralizing antibody appeared, sequential
       reisolates obtained at both early and late time after infection were
       neutralized, indicating that the neutralizing epitopes of the virus are
       conserved in the infected animals over time. This is different from the
       pathogenic SIVsm infection in macaques or HIV-1 infection in humans,
       where emergence of neutralization resistant variants seems to be the
       rule. In contrast, in HIV-2-infected macaques the biological properties
       of the virus are stable and the neutralizing antibody response shows
       extensive cross-reactivity.
 DE    Animal  Human  HIV Antibodies/*IMMUNOLOGY  HIV Antigens/BLOOD  HIV
       Infections/*ETIOLOGY  HIV Seropositivity
       HIV-2/*GENETICS/*IMMUNOLOGY/PATHOGENICITY  Leukocyte Count  Macaca
       fascicularis  Neutralization Tests  Species Specificity  Support,
       Non-U.S. Gov't  Support, U.S. Gov't, Non-P.H.S.  Time Factors  T4
       Lymphocytes/CYTOLOGY  *Variation (Genetics)  Virulence  Virus
       Replication  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).