Document 0206
 DOCN  M9460206
 TI    Epstein-Barr virus surveillance after renal transplantation.
 DT    9408
 AU    Crompton CH; Cheung RK; Donjon C; Miyazaki I; Feinmesser R; Hebert D;
       Dosch HM; Division of Nephrology, Hospital for Sick Children, Toronto,;
       Ont., Canada.
 SO    Transplantation. 1994 Apr 27;57(8):1182-9. Unique Identifier : AIDSLINE
       MED/94233599
 AB    At least 1% of organ transplant recipients develop Epstein-Barr
       virus-positive, often fatal lymphomas. EBV-positive cells accumulating
       in some organ transplant recipients were suggested to predict EBV+
       lymphoma risk but no prospective study has been reported. We used the
       polymerase chain reaction (PCR) to detect EBV genomic sequences in
       successive blood samples of 60 kidney recipients before and up to 11
       years after renal transplantation. Xenotransplantation of EBV-positive
       patient and -negative control samples into mice with severe combined
       immunodeficiency (SCID) was used to assess the tumor risk inherent in
       these samples. Despite single EBV+ cell detection sensitivity, none of
       the control samples was positive for EBV genomic sequences. In nearly
       2/3 of patients EBV genomic DNA was detectable 3-6 months after
       transplantation for about 3 months. No patient developed lymphoma.
       Lymphocytes from 8 EBV-genome positive patients and 10 healthy donors
       were engrafted into 38 SCID mice. Human B cell lymphoma developed in 75%
       of the control grafts within about 3 months. In striking contrast, none
       of the patient grafts developed lymphoma despite the large numbers of
       EBV+ cells initially transplanted. Patient lymphocyte grafts were
       resistant to injection of live EBV, while in control lymphocyte grafts
       this caused lymphoma development within 3 weeks. We conclude that a
       100-1000-fold expansion of circulating EBV+ B cell pools occurs
       frequently after organ transplantation and that it is balanced by
       effective EBV immunosurveillant functions resistant to
       immunosuppression. The mere detection of EBV genomic material was not
       predictive of lymphoma development.
 DE    Adolescence  Animal  Child  DNA, Viral/ANALYSIS  Herpesvirus 4,
       Human/GENETICS/*ISOLATION & PURIF  Human  Kidney
       Transplantation/*PATHOLOGY/PHYSIOLOGY  Lymphocytes/*MICROBIOLOGY
       Lymphoma/MICROBIOLOGY  Mice  Mice, SCID  Polymerase Chain Reaction
       Saliva/MICROBIOLOGY  Support, Non-U.S. Gov't  Time Factors
       Transplantation, Heterologous/PATHOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).