Document 0274
 DOCN  M9460274
 TI    Evidence for simian immunodeficiency virus-specific IgM and IgG response
       in peripheral blood mononuclear cells of serum enzyme-linked
       immunosorbent assay-negative nonhuman primates.
 DT    9408
 AU    Jehuda-Cohen T; Powell JD; Villinger F; Mayne AE; Sell KW; Ansari AA;
       Department of Pathology and Laboratory Medicine, Winship Cancer; Center,
       Emory University School of Medicine, Atlanta, Georgia; 30322.
 SO    J Acquir Immune Defic Syndr. 1994 Jun;7(6):539-50. Unique Identifier :
       AIDSLINE MED/94231458
 AB    In vitro polyclonal activation of peripheral blood mononuclear cells
       (PBMCs) from 70% of the simian immunodeficiency virus (SIV) serum
       enzyme-linked, immunosorbent assay (ELISA)-negative sooty mangabeys
       leads to synthesis and release of low but significant and reproducible
       levels of SIV-reactive antibodies, as determined by ELISA and Western
       blot analysis. The predominant isotype of SIV-reactive antibodies in the
       pokeweed mitogen (PWM) supernatant fluids from serum ELISA-negative
       mangabeys is IgM, whereas the predominant isotype of SIV-reactive
       antibodies in seropositive mangabeys is IgG. Depletion of CD8+ cells led
       to a marked increase in the levels of SIV-reactive antibodies detected
       in supernatant fluids from PWM-induced cultures from the serum
       ELISA-negative mangabeys. No evidence for such SIV-reactive antibodies
       has been found, to date, in similar unfractionated or CD8+
       T-cell-depleted PWM-induced PBMC cultures from uninfected macaques.
       Supernatant fluids from PWM cultures of PBMCs from a select group of
       serum ELISA-negative mangabeys, when concentrated five times, were shown
       to give a Western blot profile against SIV, similar to the profile seen
       with plasma from seropositive infected macaques and mangabeys. Evidence
       is presented to show that these serum ELISA-negative mangabeys are most
       likely latently infected with SIV. This evidence, which was obtained in
       samples from such ELISA-negative mangabeys, includes the detection of
       reverse transcriptase activity and the presence of SIV p27 in
       supernatant fluids of phytohemagglutinin-stimulated PBMCs in vitro. In
       addition, the data show the presence of CD8+ T cells that regulate
       SIV-specific Ig synthesis and show the detection of gag sequences by the
       polymerase chain reaction. Thus, the PWM assay described herein may
       provide a valuable additional tool for detection of lentivirus infection
       before or in the absence of seroconversion.
 DE    Animal  Antibodies, Viral/BIOSYNTHESIS  Antibody Specificity  Blotting,
       Western  Cells, Cultured  Cercocebus atys  Enzyme-Linked Immunosorbent
       Assay  IgG/*BIOSYNTHESIS  IgM/*BIOSYNTHESIS  Leukocytes,
       Mononuclear/IMMUNOLOGY/*MICROBIOLOGY  Macaca mulatta  Simian Acquired
       Immunodeficiency Syndrome/DIAGNOSIS/*IMMUNOLOGY  Support, Non-U.S. Gov't
       Support, U.S. Gov't, P.H.S.  SIV/*IMMUNOLOGY/PHYSIOLOGY  T-Lymphocytes,
       Suppressor-Effector/IMMUNOLOGY  Virus Latency  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).