Document 0277
 DOCN  M9460277
 TI    Brain viral burden in HIV infection.
 DT    9408
 AU    Achim CL; Wang R; Miners DK; Wiley CA; Department of Pathology,
       University of California at San Diego,; La Jolla.
 SO    J Neuropathol Exp Neurol. 1994 May;53(3):284-94. Unique Identifier :
       AIDSLINE MED/94231211
 AB    We quantitated the brain viral burden in autopsy material from AIDS
       patients with and without HIV encephalitis. Central nervous system (CNS)
       samples from 45 AIDS autopsies with less than 48 hours postmortem
       autolysis and without significant non-viral opportunistic infections
       were analyzed using immunocytochemistry (ICC), antigen capture assay
       (ACA) and polymerase chain reaction (PCR). Approximately three-quarters
       of the cases contained HIV DNA by PCR. The majority of these had
       abundant gp41 detected by ICC, but approximately one-third had no HIV
       p24 detected by ACA. With all assays, HIV proteins and DNA were most
       abundant in deep gray matter. Approximately one-quarter of the cases
       contained HIV p24 by ACA in both CNS tissue and cerebrospinal fluid. In
       more than half of the cases cytomegalovirus was detectable in the brain
       by PCR, however, only in the basal ganglia of one case was human herpes
       virus-6 detectable by PCR. In conclusion, HIV infection of the CNS was
       observed in the majority of AIDS autopsies, however, the quantity of
       virus was variable between cases and within different neuroanatomical
       regions. Differences between the quantitation methods could be either
       technical or biological, however, any of them could be used to compare
       assessment of HIV burden by different laboratories.
 DE    Base Sequence  Brain/*MICROBIOLOGY/PATHOLOGY  Cytomegalovirus/*ISOLATION
       & PURIF  Cytomegalovirus Infections/COMPLICATIONS/GENETICS/MICROBIOLOGY
       DNA, Viral/ANALYSIS  Encephalitis/ETIOLOGY/MICROBIOLOGY/PATHOLOGY
       Herpesviridae Infections/COMPLICATIONS/GENETICS/MICROBIOLOGY
       Herpesvirus 6, Human/*ISOLATION & PURIF  Human  HIV Core Protein
       p24/ANALYSIS  HIV Envelope Protein gp41/ANALYSIS  HIV
       Infections/COMPLICATIONS/GENETICS/IMMUNOLOGY/*MICROBIOLOGY/  PATHOLOGY
       Immunohistochemistry  Molecular Sequence Data  Polymerase Chain Reaction
       Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).