Document 0287
 DOCN  M9460287
 TI    B cell IL-7. Human B cell lines constitutively secrete IL-7 and express
       IL-7 receptors.
 DT    9408
 AU    Benjamin D; Sharma V; Knobloch TJ; Armitage RJ; Dayton MA; Goodwin RG;
       Division of Hematology and Oncology, Arthur G. James Cancer; Hospital,
       Columbus, OH.
 SO    J Immunol. 1994 May 15;152(10):4749-57. Unique Identifier : AIDSLINE
       MED/94230951
 AB    IL-7 was originally reported as a cytokine produced by stromal cells
       which supports pre-B cell proliferation in vitro. To determine whether
       human B cells secrete IL-7 and express IL-7R we studied a wide panel of
       B cell lines (CLS). In Northern blot analysis we detected 2.4 kb IL-7
       mRNA and by quantitative PCR we demonstrated IL-7 expression in 5 of 6 B
       CLS derived from patients with AIDS-associated Burkitt's lymphoma
       (AABCL), 3 of 3 CLS derived from patients with American Burkitt's
       lymphoma and 5 of 6 normal lymphoblastoid CLS. Only 1 of 5 African
       Burkitt's lymphoma CLS and 2 of 7 EBV- CLS expressed IL-7. A total of
       484-bp amplicons was cloned and sequenced and found to correspond to the
       original IL-7 sequence. Constitutive IL-7 secretion was detected in 5 of
       6 AABCL and in 6 of 7 normal lymphoblastoid CLS but in none of the 7
       EBV- CLS. IL-7R expression was demonstrated in 8 of 26 CLS, none of
       which secreted IL-7. Our data suggest that 1) IL-7 mRNA is expressed in
       malignant B cell phenotypes, which correspond to a narrow window in the
       B cell differentiation pathway (pre-B, early B, intermediate B), as well
       as in normal lymphoblastoid B CLS. 2) IL-7 mRNA is expressed in both
       EBV+ and EBV- CLS, but only the EBV+ CLS secrete IL-7. 3) B cells
       activated by both EBV and HIV-1 (AABCL) secrete the greatest amount of
       IL-7. 4) IL-7 autocrine loops are not evident since IL-7R were detected
       on on CLS, which do not secrete IL-7. Our data provide the first direct
       evidence of IL-7 secretion by human cells and it is yet to be determined
       whether IL-7 is secreted by other cell types.
 DE    B-Lymphocytes/CHEMISTRY/*METABOLISM/SECRETION  Base Sequence  Blotting,
       Northern  Cell Line  Human
       Interleukin-7/BIOSYNTHESIS/GENETICS/*SECRETION  Molecular Sequence Data
       Polymerase Chain Reaction  Receptors, Interleukin/*ANALYSIS  RNA,
       Messenger/ANALYSIS  Support, Non-U.S. Gov't  Support, U.S. Gov't, P.H.S.
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).