Document 0325
 DOCN  M9460325
 TI    Identification of a common clonal human immunodeficiency virus
       integration site in human immunodeficiency virus-associated lymphomas.
 DT    9408
 AU    Shiramizu B; Herndier BG; McGrath MS; Department of Pediatrics, San
       Francisco General Hospital,; University of California 94110.
 SO    Cancer Res. 1994 Apr 15;54(8):2069-72. Unique Identifier : AIDSLINE
       MED/94228518
 AB    Infection with human immunodeficiency virus type 1 (HIV-1) is associated
       with a high incidence of lymphoma. Typically, the lymphomas are B-cell
       in origin, and although they occur in the setting of HIV-1 infection,
       historical studies have found no evidence for the presence of HIV-1
       within the transformed B-cells. We describe a new class of large cell
       lymphoma wherein HIV p24 expression within the tumor specimens was found
       to be extremely high. In the first case, HIV was expressed in the
       tumor-associated transformed T-cells. In three other cases, HIV was
       found to be highly expressed in tumor-associated macrophages. These
       tumors exhibited a mixed immunophenotype histologically. Analysis by
       inverse polymerase chain reaction, using HIV long terminal repeat
       primers, demonstrated monoclonal HIV integration sites for all four
       tumors. Direct sequencing of the T-cell lymphoma inverse polymerase
       chain reaction products identified the HIV integration site within the
       fur gene, just upstream from the c-fes/fps protooncogene. Using segments
       of the fur gene as a probe, the other three monoclonal integration sites
       mapped to the same region. Although the integration and up-regulation of
       c-fes/fps was localized to the tumor cells within the T-cell lymphoma,
       the cells containing the monoclonal HIV in the other mixed
       immunophenotype lymphomas are currently unknown. These observations
       suggest that HIV may contribute directly to lymphomagenesis and identify
       a common site of HIV integration within a subset of acquired
       immunodeficiency syndrome lymphoma.
 DE    Base Sequence  DNA Primers  Human  HIV-1/*GENETICS  Immunophenotyping
       Lymphoma, AIDS-Related/*GENETICS/IMMUNOLOGY/*MICROBIOLOGY/  PATHOLOGY
       Lymphoma, B-Cell/GENETICS/MICROBIOLOGY/PATHOLOGY  Molecular Sequence
       Data  Oligonucleotide Probes  Polymerase Chain Reaction
       Protein-Tyrosine Kinase/GENETICS  Proto-Oncogene Proteins/GENETICS
       *Proto-Oncogenes  Restriction Mapping  Support, Non-U.S. Gov't  Support,
       U.S. Gov't, P.H.S.  *Virus Integration  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).