Document 0007
 DOCN  M9470007
 TI    Allergen-induced expression of cell surface markers on lymphocytes of
       Chi t I-sensitized patients.
 DT    9409
 AU    Liebers V; Raulf M; Baur X; Berufsgenossenschaftliches
       Forschungsinstitut fur Arbeitsmedizin; (BGFA), Ruhr-Universitat Bochum,
       Germany.
 SO    Allergy. 1994 Mar;49(3):163-9. Unique Identifier : AIDSLINE MED/94256625
 AB    Lymphocyte cultures of persons sensitized to the hemoglobin allergen Chi
       t I show a highly significant response to the allergen measured in the
       lymphocyte stimulation assay by (3H)-thymidine uptake. In this study, we
       investigated by flow cytometry the expression of different cell surface
       markers on lymphocytes after in vitro stimulation for 7 d with or
       without the allergen Chi t I. We determined the expression of the
       low-affinity receptor for IgE (CD23) on lymphocytes of Chi t
       I-sensitized patients and Chi t I-exposed as well as nonexposed
       controls. CD23 expression was significantly higher in patients than in
       nonexposed controls. Exposed but healthy subjects showed intermediate
       values. We also determined the expression of activation markers CD25
       (IL-2 receptor) and HLA-DR on the lymphocytes of patients and nonexposed
       controls. HLA-DR expression on non-T cells (CD3-) was significantly
       higher in patients than in controls. HLA-DR on T cells (CD3+), and CD25
       as well as CD23 expression, could be significantly enhanced after
       antigen-specific stimulation in patients but not in controls, whereas
       alpha/beta-T-cell-receptor expression was significantly reduced in
       patients. Differences between patients and controls were not observed in
       response to tetanus toxoid (TT) and phytohemagglutinin (PHA). Our
       results demonstrate antigen-specific influences on the expression of
       cell surface molecules. These findings may be valuable diagnostic
       information.
 DE    Allergens/*IMMUNOLOGY  Animal  Antigens,
       Surface/*BIOSYNTHESIS/IMMUNOLOGY  B-Lymphocytes/*IMMUNOLOGY/METABOLISM
       Cells, Cultured  Chironomidae/*IMMUNOLOGY  Comparative Study  CD4-CD8
       Ratio  Flow Cytometry  Hemoglobins/*IMMUNOLOGY  Human  HLA-DR
       Antigens/BIOSYNTHESIS  IgE/BIOSYNTHESIS  Lymphocyte Transformation
       Phytohemagglutinins/IMMUNOLOGY  Receptors, Interleukin-2/*BIOSYNTHESIS
       Support, Non-U.S. Gov't  T-Lymphocytes/*IMMUNOLOGY/METABOLISM  Tetanus
       Toxoid/IMMUNOLOGY  Time Factors  JOURNAL ARTICLE

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