Document 0009
 DOCN  M9470009
 TI    Fine T-cell subsets in alcoholics as determined by the expression of
       L-selectin, leukocyte common antigen, and beta-integrin.
 DT    9409
 AU    Cook RT; Waldschmidt TJ; Ballas ZK; Cook BL; Booth BM; Stewart BC;
       Garvey MJ; Department of Pathology, Veterans Affairs Medical Center,
       Iowa; City, IA.
 SO    Alcohol Clin Exp Res. 1994 Feb;18(1):71-80. Unique Identifier : AIDSLINE
       MED/94256602
 AB    Alcoholics admitted to the hospital solely for detoxication have been
       studied by flow cytometry to evaluate changes in the surface markers of
       peripheral blood leukocytes. As we have shown previously, such patients
       have an elevated percentage of CD8hi lymphocytes that are HLA DR+; we
       now demonstrate that they also have striking alterations in the
       quantitative relationships of the fine T-cell subsets. Both CD4+ and
       CD8hi lymphocytes have a sharply reduced percentage of the L-selectin+
       CD45RA+ subset, increased percentages of the CD45RA- subsets, and
       several other fine subset alterations. The fine subset profile suggests,
       according to current correlations of phenotype and function, that both
       CD4+ suppressor inducer and CD4-dependent CD8+ suppressor effector cells
       are reduced, whereas other subsets, including CD8+ CTL or their
       precursors, are increased in relative percentages. Some of the
       phenotypic changes are reversible over the several days following
       withdrawal. In other results, the percentage of CD8hi lymphocytes
       epxressing CD11b (beta-integrin) is shown to be reciprocal with the
       percentage expressing L-selectin both in normals and alcoholics.
       However, the regression function of CD11b vs. L-selectin on CD8hi cells
       is different for the alcoholics than for the normals, indicating an
       abnormality in the regulation of the expression of these two adhesion
       markers. Taken together, this abnormality of adhesion molecules and the
       fine subset alterations previously described indicate widespread changes
       in the peripheral lymphocytes of currently drinking alcoholics. These
       changes suggest functional deficiencies that may include alterations of
       lymphocyte traffic and other adhesion-dependent functions, and a shift
       in the balance of regulatory interactions.
 DE    Adult  Alcoholism/*IMMUNOLOGY/REHABILITATION  Antigens, CD45/*ANALYSIS
       Cell Adhesion Molecules/*ANALYSIS  CD4-CD8 Ratio/DRUG EFFECTS  Human
       Integrins/*ANALYSIS  Macrophage-1 Antigen/ANALYSIS  Male  Middle Age
       Support, Non-U.S. Gov't  Support, U.S. Gov't, Non-P.H.S.  T-Lymphocyte
       Subsets/*IMMUNOLOGY  T-Lymphocytes, Cytotoxic/DRUG EFFECTS/IMMUNOLOGY
       T-Lymphocytes, Regulatory/DRUG EFFECTS/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
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