Document 0010
 DOCN  M9470010
 TI    Effect of postnatal ethanol exposure on expression of differentiation
       antigens of murine splenic lymphocytes.
 DT    9409
 AU    Giberson PK; Blakley BR; Department of Veterinary Physiological
       Sciences, Western College; of Veterinary Medicine, University of
       Saskatchewan, Saskatoon,; Canada.
 SO    Alcohol Clin Exp Res. 1994 Feb;18(1):21-8. Unique Identifier : AIDSLINE
       MED/94256594
 AB    Ethanol is a recognized immunosuppressive agent in the chronic
       alcoholic. However, the effects of ethanol exposure on the developing
       immune system have not been extensively investigated. This study
       evaluated the effects of early postnatal ethanol exposure, via breast
       milk, on splenic lymphocyte differentiation antigen expression in
       offspring reared by ethanol-fed mice. Maternal mice were fed a liquid
       diet containing 20% ethanol-derived calories during pregnancy (E-P),
       pregnancy and lactation (E-PL), or lactation (E-L). Ad libitum-fed (C)
       and pair-fed (PF) control groups, fed a control liquid diet, were
       included. Expression of differentiation antigens on splenic lymphocytes
       from 21-day-old offspring reared by females in 1 of the 3 ethanol
       exposure conditions was evaluated by flow cytometry. Offspring reared by
       E-P females had similar numbers of splenic lymphocytes as offspring
       reared by C and pair-fed during pregnancy (PF-P) females. In contrast,
       offspring reared by E-PL and E-L females had fewer splenic lymphocytes
       than both PF-PL and PF-L (respectively), and C offspring. The number of
       Thy 1.2+, CD4+, CD8+, and IgG+ (B-cell) splenic lymphocytes was reduced
       in E-PL and E-L offspring compared with PF and C offspring. E-P
       offspring had fewer CD4+ and IgG+ splenic lymphocytes than C, but not
       PF-P, offspring. The percentage of Thy 1.2+ splenic lymphocytes was
       significantly reduced among E-PL and E-L offspring compared with PF-PL
       and PF-L (respectively), and C offspring.(ABSTRACT TRUNCATED AT 250
       WORDS)
 DE    Alcohol, Ethyl/PHARMACOKINETICS/*TOXICITY  Animal  Animals, Newborn
       Antigens, Differentiation/*ANALYSIS  B-Lymphocytes/*DRUG
       EFFECTS/IMMUNOLOGY  CD4-CD8 Ratio  Female  Fetal Alcohol
       Syndrome/*IMMUNOLOGY  Flow Cytometry  Killer Cells, Natural/DRUG
       EFFECTS/IMMUNOLOGY  Leukocyte Count  Male  Rats  Support, Non-U.S. Gov't
       T-Lymphocytes/*DRUG EFFECTS/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).