Document 0011
 DOCN  M9470011
 TI    Flow cytometric analysis of lymphocyte subsets of mice maintained on an
       ethanol-containing liquid diet.
 DT    9409
 AU    Hsiung L; Wang J; Waltenbaugh C; Department of Microbiology-Immunology,
       Northwestern University; Medical School, Chicago, IL 60611.
 SO    Alcohol Clin Exp Res. 1994 Feb;18(1):12-20. Unique Identifier : AIDSLINE
       MED/94256579
 AB    Alcoholic patients often have impaired immune function, yet little is
       known about the precise mechanism(s) of this impairment. We have
       previously shown that ethanol consumption by mice alters
       copolymer-specific humoral and cellular immune responses. In this study,
       we asked whether alcohol consumption by mice would phenotypically alter
       lymphocyte populations. Female C57BL/6 mice were fed a nutritionally
       complete liquid diet containing 35% ethanol-derived calories for up to 8
       days. As controls, mice either were fed a liquid control diet that
       isocalorically substitutes sucrose for ethanol or remained on a standard
       solid diet and water ad libitum. Although mice fed ethanol-containing
       liquid or pair-fed control liquid diets have decreased numbers of spleen
       cells compared with solid diet controls, only the ethanol-containing
       diet allowed normally nonresponder C57BL/6 spleen cells to make antibody
       responses to the poly(Glu50Tyr50) synthetic copolymer antigen. Flow
       cytometric analysis of splenic lymphocyte populations of mice on the
       ethanol-containing diet shows an increase in the relative proportion of
       T-lymphocytes as compared with mice on either solid or liquid control
       diets. No such change is seen for either B-cell or natural killer cell
       populations in these same mice. Both liquid control and liquid ethanol
       diets caused a slight decrease in the CD4:CD8 ratios of splenic
       T-lymphocytes. We see the relative percentage of T-cells bearing the
       alpha beta T-cell receptor (TcR) increases in the spleens of liquid
       ethanol diet mice; a smaller increase TcR alpha beta usage is seen in
       the spleens of liquid control mice, compared with solid diet
       mice.(ABSTRACT TRUNCATED AT 250 WORDS)
 DE    Alcohol, Ethyl/*TOXICITY  Alcoholism/*IMMUNOLOGY  Animal  B-Lymphocyte
       Subsets/*DRUG EFFECTS/IMMUNOLOGY  CD4-CD8 Ratio/DRUG EFFECTS  Female
       *Flow Cytometry  Leukocyte Count/DRUG EFFECTS  Mice  Mice, Inbred C57BL
       Peptides/IMMUNOLOGY  Receptors, Antigen, T-Cell, alpha-beta/ANALYSIS
       Spleen/IMMUNOLOGY  Support, U.S. Gov't, P.H.S.  T-Lymphocyte
       Subsets/*DRUG EFFECTS/IMMUNOLOGY  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).