Document 0038
 DOCN  M9470038
 TI    TAR RNA-binding protein is an inhibitor of the interferon-induced
       protein kinase PKR.
 DT    9409
 AU    Park H; Davies MV; Langland JO; Chang HW; Nam YS; Tartaglia J; Paoletti
       E; Jacobs BL; Kaufman RJ; Venkatesan S; Laboratory of Molecular
       Microbiology, National Institute of; Allergy and Infectious Diseases,
       Bethesda, MD 20892.
 SO    Proc Natl Acad Sci U S A. 1994 May 24;91(11):4713-7. Unique Identifier :
       AIDSLINE MED/94255397
 AB    A cDNA encoding a double-stranded-RNA (dsRNA)-binding protein was
       isolated by screening a HeLa cell cDNA expression library for proteins
       that bind the HIV-1 Rev-responsive-element RNA. The cDNA encoded a
       protein that was identical to TRBP, the previously reported cellular
       protein that binds the transactivation response element (TAR) RNA of
       human immunodeficiency virus type 1. TRBP inhibited phosphorylation of
       the interferon-induced ribosome-associated protein kinase PKR and of the
       eukaryotic translation initiation factor eIF-2 alpha in a
       transient-expression system in which the translation of a reporter gene
       was inhibited by the localized activation of PKR. TRBP expression in
       HeLa cells complemented the growth and protein-synthesis defect of a
       vaccinia virus mutant lacking the expression of the dsRNA-binding
       protein E3L. These results implicate TRBP as a cellular regulatory
       protein that binds RNAs containing specific secondary structure(s) to
       mediate the inhibition of PKR activation and stimulate translation in a
       localized manner.
 DE    eIF-2/METABOLISM  Animal  Bacterial Proteins/*METABOLISM  Cell Line
       Enzyme Induction  Genetic Complementation Test  Hela Cells  Human
       Interferons/*PHARMACOLOGY  Membrane Proteins/*METABOLISM  Phenotype
       Phosphorylation  Protein-Serine-Threonine Kinases/*ANTAGONISTS & INHIB/
       BIOSYNTHESIS  RNA-Binding Proteins/*METABOLISM  Support, U.S. Gov't,
       P.H.S.  Vaccinia Virus/GENETICS/PHYSIOLOGY  Virus Replication/GENETICS
       JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).