Document 0062
 DOCN  M9470062
 TI    HIV-1/HIV-2 seronegativity in HIV-1 subtype O infected patients [see
       comments]
 DT    9409
 AU    Loussert-Ajaka I; Ly TD; Chaix ML; Ingrand D; Saragosti S; Courouce AM;
       Brun-Vezinet F; Simon F; Virology Department, Bichat-Claude Bernard
       Hospital, Paris,; France.
 SO    Lancet. 1994 Jun 4;343(8910):1393-4. Unique Identifier : AIDSLINE
       MED/94254523
 CM    Comment in: Lancet 1994 Jun 4;343(8910):1376
 AB    Nine patients with atypical HIV-1 western blot profiles were diagnosed
       as having HIV-1 subtype O infection. All the patients were living in
       France; eight originated from Cameroon and one from France. Lymphocyte
       DNA amplification by the polymerase chain reaction was only positive
       when HIV-1 subtype O specific primers were used. Preliminary sequence
       analysis of amplified products and serological reactivity against a
       specific subtype O synthetic env peptide confirmed HIV-1 subtype O
       infection. HIV-1/HIV-2 enzyme-linked immunosorbent assays, especially
       those based on env peptides or on the sandwich format, can be negative
       in HIV-1 subtype O infection.
 DE    Acquired Immunodeficiency Syndrome/DIAGNOSIS/*MICROBIOLOGY  Adult
       Blotting, Western  Cameroon  Enzyme-Linked Immunosorbent Assay  France
       Gene Amplification  Human  *HIV Seronegativity
       HIV-1/*CLASSIFICATION/GENETICS/IMMUNOLOGY
       HIV-2/*CLASSIFICATION/GENETICS/IMMUNOLOGY  Middle Age  Polymerase Chain
       Reaction  JOURNAL ARTICLE

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