Document 0064
 DOCN  M9470064
 TI    Screening anti-HIV Chinese materia medica with HIV and equine infectious
       anemic virus reverse transcriptase.
 DT    9409
 AU    Tang X; Chen H; Zhang X; Quan K; Sun M; Institute of Medical
       Biotechnology, CAMS, Beijing.
 SO    J Tradit Chin Med. 1994 Mar;14(1):10-3. Unique Identifier : AIDSLINE
       MED/94254497
 AB    The inhibitory activities of human immunodeficiency virus reverse
       transcriptase (HIV-RT) inhibitors PFA and Suramin against HIV-RT and
       equine infectious anemic virus reverse transcriptase (EIAV-RT) were
       studied in this paper. The 50% inhibitory concentration (IC50) of HIV-RT
       and EIAV-RT treated by PFA and Suramin were 0.2 mumol, 9.8 mumol and 17
       mumol, 19.9 mumol, respectively. More than thirty Chinese medicines,
       including recipes, herbs, extracts of traditional materia medica and
       isolated compounds were tested using HIV-RT and EIAV-RT as target
       enzymes. It was found that 5 crude extracts such as Rhizoma Polygoni
       Cuspidati, and Radix Notoginseng, 7 isolated compounds like Flavone of
       Ramulus Visci, Flavone of Ajuga Decumbens Thumb, and Aristolochic acid,
       as well as the extract of the complex prescription Xiao Chai Hu Tang
       have shown various inhibitory actions on these two enzymes. The
       activities of the two enzymes were comparable, but HIV-RT was more
       sensitive, suggesting that HIV-RT can be used as index for the screening
       of anti-aids drugs.
 DE    Animal  Antiviral Agents/*PHARMACOLOGY  Drug Screening  Drugs, Chinese
       Herbal/*PHARMACOLOGY  HIV/*DRUG EFFECTS/ENZYMOLOGY  Infectious Anemia
       Virus, Equine/*DRUG EFFECTS/ENZYMOLOGY  Reverse Transcriptase/*DRUG
       EFFECTS/METABOLISM  Suramin  JOURNAL ARTICLE

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