Document 0100
 DOCN  M9470100
 TI    Bias of culture techniques for diagnosing mixed Mycobacterium genavense
       and Mycobacterium avium infection in AIDS.
 DT    9409
 AU    Kirschner P; Vogel U; Hein R; Bottger EC; Institut fur Medizinische
       Mikrobiologie, Medizinische Hochschule; Hannover, Germany.
 SO    J Clin Microbiol. 1994 Mar;32(3):828-31. Unique Identifier : AIDSLINE
       MED/94253357
 AB    Disseminated Mycobacterium avium infection is a common complication in
       late-stage AIDS. We describe a patient seropositive for human
       immunodeficiency virus with a disseminated infection caused by
       mycobacteria. Acid-fast rods were visible by microscopy in stool and
       bone marrow and in specimens from skin, liver, spleen, lung, and lymph
       nodes. Using molecular biology techniques and standard culture
       techniques, we found evidence of a mixed mycobacterial infection with M.
       genavense and M. avium. We suggest that the prevalence of M. genavense
       infection in patients with AIDS is underestimated because of the bias
       toward M. avium when using standard techniques for the detection and
       identification of mycobacteria.
 DE    Adult  AIDS-Related Opportunistic Infections/COMPLICATIONS/*DIAGNOSIS
       *Bacteriological Techniques/STATISTICS & NUMER DATA  Base Sequence  Bias
       (Epidemiology)  Case Report  Comparative Study  DNA Primers/GENETICS
       DNA, Bacterial/GENETICS  DNA, Ribosomal/GENETICS  Human  HIV-1  Male
       Molecular Sequence Data  Mycobacterium/GENETICS/ISOLATION & PURIF
       Mycobacterium avium Complex/GENETICS/ISOLATION & PURIF  Mycobacterium
       avium-intracellulare Infection/COMPLICATIONS/  *DIAGNOSIS  Mycobacterium
       Infections/COMPLICATIONS/*DIAGNOSIS  Polymerase Chain Reaction  Support,
       Non-U.S. Gov't  JOURNAL ARTICLE

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