Document 0101
 DOCN  M9470101
 TI    Detection of anti-human immunodeficiency virus type 1 (HIV-1)
       immunoglobulin G in urine by an ultrasensitive enzyme immunoassay
       (immune complex transfer enzyme immunoassay) with recombinant reverse
       transcriptase as an antigen.
 DT    9409
 AU    Hashinaka K; Hashida S; Hirota K; Saitoh A; Nakata A; Shinagawa H; Oka
       S; Shimada K; Ishikawa E; Department of Biochemistry, Medical College of
       Miyazaki, Japan.
 SO    J Clin Microbiol. 1994 Mar;32(3):819-22. Unique Identifier : AIDSLINE
       MED/94253354
 AB    Anti-human immunodeficiency virus type 1 immunoglobulin G in urine was
       detected by an immunoassay with reverse transcriptase as the antigen and
       beta-D-galactosidase as the label; this immunoassay was 30-fold more
       sensitive than the previous immunoassay with peroxidase as the label.
       The sensitivity and specificity were both 100%. The lowest signal for
       asymptomatic carriers was 20-fold higher than the highest signal for
       seronegative subjects.
 DE    beta-Galactosidase  Adolescence  Adult  Aged  Antigens  Evaluation
       Studies  Human  HIV Antibodies/*URINE  HIV
       Seropositivity/DIAGNOSIS/IMMUNOLOGY  HIV-1/*IMMUNOLOGY  IgG/URINE
       *Immunoenzyme Techniques/STATISTICS & NUMER DATA  Middle Age
       Recombinant Proteins/IMMUNOLOGY  Reverse Transcriptase/IMMUNOLOGY
       Sensitivity and Specificity  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).