Document 0130
 DOCN  M9470130
 TI    HIV-1 reactivation after an oxidative stress mediated by different
       reactive oxygen species.
 DT    9409
 AU    Piette J; Legrand-Poels S; Laboratory of Fundamental Virology,
       University of Liege,; Belgium.
 SO    Chem Biol Interact. 1994 Jun;91(2-3):79-89. Unique Identifier : AIDSLINE
       MED/94251850
 AB    An important aspect of infection by the human immunodeficiency virus
       (HIV-1) type 1 is its long clinical latency period, suggesting that the
       provirus may remain latent for extended periods of time after primary
       infection. Numerous factors such as cytokines, tumor promoters,
       co-infection by several viruses and physical agents are able to
       reactivate latent virus. Since a common denominator, shared by several
       of these agents, is their ability to cause stress conditions, we have
       examined the effects of an oxidative stress mediated by reactive oxygen
       species on HIV-1 latently infected monocytes (U1) or lymphocytes
       (ACH-2). Exposure of these two cell lines to hydrogen peroxide causes a
       decrease of cell viability but among the cells surviving the treatment,
       a HIV-1 reactivation can be observed as measured by increased RT
       activities depicted in cell supernatants or by the appearance of HIV-1
       antigens inside cells. Singlet oxygen (1O2) when generated either in the
       cytoplasm or in the cell nucleus can also promote an important HIV-1
       reactivation from treated cells. However, extracellular generation of
       1O2 cannot trigger the HIV-1 reactivation although this kind of
       treatment is highly cytotoxic. These experiments demonstrate that
       different reactive oxygen species are able to lead to an intracellular
       pro-oxidant state initiating one or several signalling pathways which
       lead in fine to the HIV-1 LTR transactivation by regulatory proteins.
 DE    Cell Line  Cell Survival/DRUG EFFECTS  Human  Hydrogen
       Peroxide/PHARMACOLOGY  HIV-1/DRUG EFFECTS/*PHYSIOLOGY  Monocytes/DRUG
       EFFECTS/*MICROBIOLOGY  Oxidation-Reduction  Oxygen/PHARMACOLOGY
       Reactive Oxygen Species/*PHARMACOLOGY  Support, Non-U.S. Gov't
       T-Lymphocytes/DRUG EFFECTS/*MICROBIOLOGY  *Virus Activation  *Virus
       Latency  JOURNAL ARTICLE  REVIEW  REVIEW, TUTORIAL

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).