Document 0141
 DOCN  M9470141
 TI    Effect of human immunodeficiency virus type 1 (HIV-1) monocyte-derived
       macrophages infection on the manganous superoxide dismutase gene
       expression.
 DT    9409
 AU    Raoul H; Mabondzo A; Le Naour R; Dormont D; Laboratoire de
       Neuropathologie experimentale et Neurovirologie,; CRSSA/DSV/DPTE,
       Commissariat a l'Energie Atomique, Fontenay aux; Roses, France.
 SO    Chem Biol Interact. 1994 Jun;91(2-3):123-31. Unique Identifier :
       AIDSLINE MED/94251838
 AB    Clinical and biological features indicate that a dysregulation of
       microbicidal activity occurs in the cells of mononuclear phagocytic
       lineage of HIV-1-infected patients. Thus, the regulation of MnSOD gene
       expression has been investigated during the 10 h following in vitro HIV
       macrophage infection. As previously reported, in HIV-1 LAI-infected
       macrophages a high expression of the MnSOD gene is observed 2 and 4 h
       after infection. These results are confirmed when cells are infected
       with three macrophage-tropic strains HIV-1, DAS, PAR and Bal. Moreover,
       the detection of the MnSOD gene expression in the macrophage cultures is
       associated with the cellular tropism of the viral strains used. The
       binding of recombinant GP160 by itself is not sufficient to induce MnSOD
       expression. In fact, the same MnSOD gene induction was obtained with the
       heat inactivated viral isolates, indicating that these phenomena are due
       to the viral entry. On the other hand, phagocytosis of latex beads
       triggers a high expression of the MnSOD gene in macrophages, showing
       that phagocytosis of HIV may be sufficient to induce the expression of
       that gene. Taken together, these results indicate that the MnSOD gene
       expression observed within 10 h following infection of macrophages is
       mainly related to membrane biophysical unspecific modifications.
 DE    Blotting, Northern  Cells, Cultured  Endocytosis  *Gene Expression
       Regulation, Enzymologic  Gene Products, env/METABOLISM  Human
       HIV-1/*PHYSIOLOGY  Macrophages/ENZYMOLOGY/*MICROBIOLOGY  Monocytes
       Protein Precursors/METABOLISM  Recombinant Proteins/METABOLISM
       Superoxide Dismutase/BIOSYNTHESIS/*GENETICS  Support, Non-U.S. Gov't
       Transcription, Genetic  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).