Document 0818
 DOCN  M9480818
 TI    Comparison of direct fluorescence antibody and calcofluor white stain
       for detection of Pneumocystis carinii in respiratory specimens.
 DT    9410
 AU    Aslanzadeh J; Kowalski I; Stelmach PS; Ryan RW; University of
       Connecticut School of Medicine, Farmington.
 SO    Abstr Gen Meet Am Soc Microbiol. 1994;94:625 (abstract no. V-47). Unique
       Identifier : AIDSLINE ASM94/94313127
 AB    Direct fluorescence monoclonal antibody stain (DFA) was compared,
       prospectively, with calcofluor white stain (CFW) for diagnosis of
       Pneumocystis carinii in 163 respiratory specimens from 97 patients. The
       patient population included HIV infection (58%), bone marrow transplant
       recipient (10%), immunosuppressed due to chemotherapy (21%) and others
       (11%). Nineteen specimens, 12 sputa, 6 BALs, 1 induced sputum, were
       positive by DFA. In contrast, only 6 sputa, 5 BALs were positive if one
       CFW stained slide was examined per specimen. However, an additional BAL
       was positive if two slides were examined per specimen. All specimen
       positive by CFW were also positive by DFA. Of 86 Sputa that were
       negative by either methods 3 specimens were followed by induced sputum
       18 by BAL 6 by lung biopsy and 2 by pleural fluid. All the subsequent
       induced sputa, pleural fluids, and lung biopsies were negative by both
       methods. However, 4 of 18 subsequent BALs (22%) were positive by both
       methods provided at least two CFW stained slides were examined per
       specimen. We conclude that, except for expectorated sputum, CFW is a
       rapid and inexpensive test to detect P. carinii from most respiratory
       specimens.
 DE    AIDS-Related Opportunistic Infections/*DIAGNOSIS  *Benzenesulfonates
       Biopsy  Bone Marrow Transplantation  Bronchoalveolar Lavage
       Fluid/*MICROBIOLOGY  Comparative Study  *Fluorescent Antibody Technique
       Human  HIV Infections/COMPLICATIONS  Immunosuppression/ADVERSE EFFECTS
       Lung/*MICROBIOLOGY/PATHOLOGY  Pleural Effusion/MICROBIOLOGY
       Pneumocystis carinii Infections/*DIAGNOSIS/ETIOLOGY  Postoperative
       Complications/DIAGNOSIS/MICROBIOLOGY  Prospective Studies
       Sputum/*MICROBIOLOGY  Stains and Staining/METHODS  MEETING ABSTRACT

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