Document 0822
 DOCN  M9480822
 TI    Detection of JC virus DNA by PCR in brain tissue and cerebrospinal fluid
       from HIV infected patients.
 DT    9410
 AU    Caldarelli Stefano R; Omodeo Zorini E; Rivolta GE; Achilli G; Cattaneo
       E; Vago L; Boldorini R; Ferrante P; Biology Lab., Fondazione Don C.
       Gnocchi IRCCS, Milano, Italy.
 SO    Abstr Gen Meet Am Soc Microbiol. 1994;94:623 (abstract no. V-36). Unique
       Identifier : AIDSLINE ASM94/94313123
 AB    The correct diagnosis of progressive multifocal leukoencephalopathy
       (PML), a severe demyelinating disease caused from JC Virus, is of
       particular relevance in AIDS patients. For these reasons we set a
       Polymerase Chain Reaction (PCR) method to detect JCV DNA both in brain
       tissues collected at autopsy from 17 cases and in cerebrospinal fluid
       (CSF) and urine of 7 AIDS patients with different neurological symptoms,
       including a suspected PML case. The genomic region chosen for PCR
       belongs to the Large T antigen of JCV and contains a Bam HI site that is
       present only in JCV but not in BKV genome. JCV DNA has been detected in
       7 (58.3%) paraffin-embedded, in 2 (66.6%) formalin-fixed, in 2 (100%)
       frozen tissues and in 2 (100%) frozen CSF. Regarding the 7
       neurologically ill patients only one, presenting clinical and
       radiological PML signs, was positive both in CSF and urine for JCV DNA.
       Our results indicate that PCR is useful in the diagnosis of PML at
       autopsy but mainly that it is efficient, specific and practical for the
       correct diagnosis and follow-up of living AIDS patients.
 DE    Autopsy  AIDS-Related Opportunistic Infections/CEREBROSPINAL FLUID/
       *DIAGNOSIS/URINE  Brain/*MICROBIOLOGY  DNA,
       Viral/*ANALYSIS/CEREBROSPINAL FLUID/URINE  Follow-Up Studies  Human
       Papovaviridae Infections/COMPLICATIONS/*DIAGNOSIS  Polymerase Chain
       Reaction/*METHODS  Polyomavirus hominis 2/*ISOLATION & PURIF
       Sensitivity and Specificity  Tumor Virus
       Infections/COMPLICATIONS/*DIAGNOSIS  MEETING ABSTRACT

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