Document 0823
 DOCN  M9480823
 TI    Rapid identification of Pneumocystis carinii isolates by random
       amplification polymorphic DNA fingerprinting.
 DT    9410
 AU    Chary-Reddy S; Graves DC; Univ. of Okla. Hlth. Sci. Ctr., Oklahoma City.
 SO    Abstr Gen Meet Am Soc Microbiol. 1994;94:622 (abstract no. V-27). Unique
       Identifier : AIDSLINE ASM94/94313122
 AB    Pneumocystis carinii is an opportunistic pathogen that causes pneumonia
       in immunocompromised hosts including humans. It is the leading cause of
       morbidity and mortality in patients with AIDS. To understand more
       clearly the transmission of this organism, it is important to develop
       methods that can distinguish P. carinii isolates. We utilized a randomly
       designed primer P14 (GCA CAC TCT C) and the random amplified polymorphic
       DNA (RAPD) assay (Williams et al. 1990. Nucleic acids Res. 18: 6531) to
       amplify genomic DNA segments of P. carinii obtained from rat, mouse,
       horse, ferret and human. The amplified products were detected by gel
       electrophoresis and Southern hybridization using a 32P labelled
       repetitive DNA sequence from rat P. carinii. Genomic DNA from P. carinii
       isolates of different species gave distinct RAPD profiles, suggesting
       that these immunocompromised hosts were infected by genetically distinct
       P. carinii strains. Genomic DNA of P. carinii obtained from two
       different strains of rats (Sprague-Dawley from Sasco, Inc. and Wistar
       from Hilltop laboratories) and Sprague-Dawley rats from different
       vendors produced different band patterns. Furthermore, we were able to
       demonstrate two different genetic variants of P. carinii in Wistar rats
       suggesting a coinfection. In preliminary studies using DNA obtained from
       two different human P. carinii isolates, identical RAPD profiles were
       observed. Thus, it appears that the RAPD assay using primer P14 is an
       attractive method for identifying specific strains of P. carinii and is
       suitable for large scale epidemiological studies.
 DE    Animal  AIDS-Related Opportunistic Infections/*DIAGNOSIS  Base Sequence
       Blotting, Southern/METHODS  Comparative Study  DNA Primers  Ferrets
       Gene Amplification/*METHODS  Horses  Human  Mice  Molecular Sequence
       Data  Pneumocystis carinii/GENETICS/*ISOLATION & PURIF  Pneumocystis
       carinii Infections/*DIAGNOSIS  Polymorphism (Genetics)  Random
       Allocation  Rats  Rats, Sprague-Dawley  Rats, Wistar  Repetitive
       Sequences, Nucleic Acid  Species Specificity  Variation (Genetics)
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).