Document 0826
 DOCN  M9480826
 TI    Evaluation of the MicroTrak II HIV-1/HIV-2 recombinant antigen enzyme
       immunoassay.
 DT    9410
 AU    Kline R; Newhouse R; Granade T; Phillips S; Moss M; Quinn TC; Johns
       Hopkins Univ., Baltimore, MD.
 SO    Abstr Gen Meet Am Soc Microbiol. 1994;94:622 (abstract no. V-28). Unique
       Identifier : AIDSLINE ASM94/94313119
 AB    In a study, the reliability, sensitivity, and specificity of the
       MicroTrak II HIV-1/HIV-2 EIA (MT II) were compared to licensed EIAs and
       Western Blot (WB). 2490 sera from various populations in the US, Africa,
       and India were evaluated at 3 different sites using the MT II and
       licensed HIV-1/HIV-2 EIAs (Genetic Systems or Abbott). All repeatedly
       reactive sera were reconfirmed by WB for HIV-1. Sera that were
       indeterminate by WB, had discrepant results, or were previously known to
       be HIV-2 positive were retested using a licensed HIV-2 EIA (Genetic
       Systems). All reactive samples were confirmed by HIV-2 WB. Discrepant
       samples were also tested using a peptide assay (Select HIV). Final
       interpretation of serological results was primarily based on WB and, in
       some cases, RIPA, PCR, and culture. WB and other confirmatory testing
       identified 910 HIV-1/HIV-2 positive samples and 1580 HIV-1/HIV-2
       negative samples. MT II detected all 910 positive samples for a
       sensitivity of 100% and the licensed HIV-1/HIV-2 EIAs detected 904
       positive samples for a sensitivity of 99.3%. MT II characterized 1574
       negative samples as negative for a specificity of 99.6% and the licensed
       EIAs detected 1565 negative samples as negative for a specificity of
       99.1%. The 6 false positives by MT II were all sera from Africa. The
       MicroTrak II HIV-1/HIV-2 EIA is a reliable assay that has a slightly
       higher sensitivity and specificity compared to other licensed EIAs.
 DE    Africa  Blotting, Western/METHODS  Comparative Study  False Positive
       Reactions  Human  HIV Antigens/*BLOOD  HIV
       Seropositivity/BLOOD/*DIAGNOSIS  HIV-1/*ISOLATION & PURIF
       HIV-2/*ISOLATION & PURIF  *Immunoenzyme Techniques  India  Predictive
       Value of Tests  Sensitivity and Specificity  United States  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).