Document 0850
 DOCN  M9480850
 TI    Feline bone marrow stromal fibroblasts are a target of feline
       immunodeficiency virus infection in vitro and in vivo.
 DT    9410
 AU    Lu YS; Ho SI; Read RW; Richardson JA; Pakes SP; University of Texas
       Southwestern Medical Center, Dallas.
 SO    Abstr Gen Meet Am Soc Microbiol. 1994;94:485 (abstract no. T-18). Unique
       Identifier : AIDSLINE ASM94/94313095
 AB    Similar to HIV infection in humans, cats infected with feline
       immunodeficiency virus (FIV) develop anemia, neutropenia and
       lymphopenia. These hematologic abnormalities are thought to be due to
       altered marrow micro-environments because of infected marrow stromal
       cells. Susceptibility of marrow stromal cells to FIV infection is
       unknown. Stromal fibroblasts from bone marrow cells isolated from three
       SPF cats were grown in culture medium and were exposed to FIV (Petaluma
       strain). Portions of the FIV-exposed stromal fibroblasts were cocultured
       with CRFK cells to amplify FIV infection. Culture supernatants and cells
       from amplified and nonamplified cultures were assayed for viral
       infection. Results show that cells from both cultures are positive for
       proviral DNA by PCR and viral antigens by IFA. However, p26 viral
       antigen (ELISA assay) is found only in supernatant of stromal
       fibroblasts cocultured with CRFK cells. Similarly, marrow stromal
       fibroblasts from three FIV-infected cats were prepared and treated in
       the same manner without exposure to FIV in vitro. Some of the stromal
       fibroblasts were cocultured with CRFK cells or Con A-activated
       peripheral blood lymphoblasts from an SPF cat. The results show that
       proviral DNA and viral antigens are positive in stromal fibroblasts and
       cocultured cells. However, p26 viral antigen is detected only in
       supernatant of stromal fibroblasts of individual cat cocultured with
       CRFK or activated lymphoblasts, but not both. Both in vitro and in vivo
       results indicate that feline marrow stromal fibroblasts are a target of
       FIV infection. FIV-infected cats may serve a good model to study
       hematopoietic dysfunction of HIV infection in humans.
 DE    Animal  Antigens, Viral/ANALYSIS  Bone Marrow/*CYTOLOGY/MICROBIOLOGY
       Cats  Cells, Cultured  Comparative Study  DNA, Viral/ANALYSIS
       Enzyme-Linked Immunosorbent Assay  Feline Acquired Immunodeficiency
       Syndrome/*PHYSIOPATHOLOGY  Fibroblasts/CYTOLOGY/MICROBIOLOGY
       Hematopoiesis  Human  HIV Infections/PHYSIOPATHOLOGY  Immunodeficiency
       Virus, Feline/*GROWTH & DEVELOPMENT/GENETICS/  ISOLATION & PURIF
       Polymerase Chain Reaction  Proviruses/GROWTH &
       DEVELOPMENT/GENETICS/ISOLATION & PURIF  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).