Document 0859
 DOCN  M9480859
 TI    Role of Ca(++)-dependent and Ca(++)-independent protein kinase C
       isozymes on activation of HIV-1.
 DT    9410
 AU    Kim C; Lim S; Gollapudi S; Gupta S; University of California, Irvine.
 SO    Abstr Gen Meet Am Soc Microbiol. 1994;94:483 (abstract no. T-6). Unique
       Identifier : AIDSLINE ASM94/94313086
 AB    Protein kinase C (PKC) plays a crucial role in HIV-1 replication.
       Because of the recognized molecular and biochemical heterogeneity of
       PKC, we have studied the effects of various PKC isozyme agonists on
       HIV-1 activation in chronically infected promonocytic U1 cells that
       produce minimal or no virus. U1 cells were incubated with various
       concentrations of 12-deoxyphorbol 13-phenylacetate (dPP),
       12-deoxyphorbol 13-phenylacetate 20-acetate (dPPA), and thymeleatoxin
       (TT) for various time periods and viral production was measured by
       reverse transcriptase and HIV-1 p24 antigen ELISA assays. dPP, a broad
       PKC isozyme agonist of both Ca(++)-dependent (PKC alpha, PKC beta, and
       PKC gamma) and Ca(++)-independent PKC isozymes (PKC delta, PKC epsilon,
       and PKC zeta), and TT, an agonist of Ca(++)-dependent PKC isozymes, in a
       concentration dependent manner induced HIV-1 production. Whereas
       12-deoxyphorbol 13-phenylacetate 20-acetate (dPPA), a PKC beta I isozyme
       agonist had minimal effect on viral production at these concentration.
       This study demonstrates that activation of both Ca(++)-dependent and
       Ca(++)-independent PKC isozymes play a role in the activation of latent
       HIV-1. Furthermore, PKC beta I appears to have minimal, if any, role in
       HIV-1 activation.
 DE    Calcium/*METABOLISM/PHARMACOLOGY  Cell Line  Comparative Study
       Enzyme-Linked Immunosorbent Assay  Human  HIV Core Protein
       p24/ANALYSIS/BIOSYNTHESIS  HIV-1/*GROWTH & DEVELOPMENT
       Isoenzymes/*METABOLISM  Phorbol Esters/PHARMACOLOGY  Protein Kinase
       C/*METABOLISM  *Virus Activation  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).