Document 0861
 DOCN  M9480861
 TI    A rapid sensitive, solution based fluorescent in situ hybridization
       (FISH) assay for the detection of HIV mRNA in infected cell lines by
       multi-colored flow cytometry.
 DT    9410
 AU    Poindexter B; Cubbage M; Weber W; Ku J; Bresser J; Aprogenex, Inc.,
       Houston, TX.
 SO    Abstr Gen Meet Am Soc Microbiol. 1994;94:483 (abstract no. T-3). Unique
       Identifier : AIDSLINE ASM94/94313084
 AB    Rapid detection of low levels of HIV infected cells is necessary for the
       screening of donated blood as well as in the clinical diagnosis of
       infection. Aprogenex has developed an HIV diagnostic test, Virafy,
       sensitive enough to detect infected cell lines diluted down to 1 in
       10,000 with uninfected cell lines by flow cytometry. The test can be
       performed in less than 1 hour and with the use of proprietary background
       reducers and signal enhancers these low levels of detection can be
       obtained. Other potential applications include determination of the
       level of RNA infection in cells as well as the evaluation of therapeutic
       regimens.
 DE    Flow Cytometry/*METHODS  Human  HIV/GENETICS/*ISOLATION & PURIF  In Situ
       Hybridization, Fluorescence/*METHODS  Reagent Kits, Diagnostic  RNA,
       Messenger/*ANALYSIS  Sensitivity and Specificity  MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).