Document 0863
 DOCN  M9480863
 TI    Identification of HIV-1 variants transmitted from mothers to fetus.
 DT    9410
 AU    Furtado MR; Hoeft J; Garcia PM; Department of Pathology, Northwestern
       University, Chicago, IL; 60611.
 SO    Abstr Gen Meet Am Soc Microbiol. 1994;94:482 (abstract no. T-2). Unique
       Identifier : AIDSLINE ASM94/94313082
 AB    Identification of HIV-1 sequence variants that are transmitted to
       infants and determination of the timing of transmission are crucial to
       understanding the underlying mechanisms. We analyzed fetal tissue from
       five abortuses and detected HIV-1 DNA in four. Analysis for maternal DNA
       in the fetal samples by PCR amplification of the apo-B VNTR locus
       detected contaminating DNA in one of the samples. The C2V3 to V4V5
       region of HIV-1 envelope gene was amplified from three maternal PBMC
       samples and corresponding fetal tissue, inserted into plasmid vectors
       and sequenced. Phylogenetic analysis of the sequence variants indicated
       that viral sequences found in fetal tissue exhibited a low degree of
       divergence and were highly homologous to some of the variants in the
       mother. Our studies suggest that transmission could occur early in
       gestation.
 DE    Abortion  Acquired Immunodeficiency Syndrome/*TRANSMISSION  Female
       Fetus/*MICROBIOLOGY  Human  HIV-1/*GENETICS/ISOLATION & PURIF
       Polymerase Chain Reaction  Pregnancy  Pregnancy Complications,
       Infectious/*MICROBIOLOGY  Support, Non-U.S. Gov't  *Variation (Genetics)
       Viral Envelope Proteins/GENETICS  MEETING ABSTRACT

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