Document 0873
 DOCN  M9480873
 TI    Isolation of fastidious mycobacteria from patients with AIDS.
 DT    9410
 AU    Yajko DM; Clancy MN; Goodman C; Hadley WK; Univ. of California, San
       Francisco General Hospital.
 SO    Abstr Gen Meet Am Soc Microbiol. 1994;94:180 (abstract no. U-45). Unique
       Identifier : AIDSLINE ASM94/94313072
 AB    During a recent 14 month period (6/92-8/93) slow-growing fastidious
       mycobacteria were recovered at San Francisco General Hospital from 22
       specimens submitted from 10 patients with AIDS. Twenty of the isolates
       (from 9 patients) were recovered from blood and two isolates (from a
       single patient) were recovered from AFB smear positive sputum specimens.
       Nineteen of the blood isolates (from 8 patients) grew in Bactec 12B
       broth with a median time to detection of 42 days (range 14-54 days).
       Three isolates (a blood isolate from one patient and the two sputum
       isolates from another patient) did not grow in Bactec 12B broth but were
       seen on Middlebrook 7H11 agar after 49-54 days of incubation. None of
       the isolates grew on Lowenstein-Jensen medium but in most cases colonies
       were seen on the primary non-selective 7H11 agar plate or on the first
       sub-culture from 12B broth to 7H11 agar. Further sub-culture to solid
       media (7H11 agar, 7H11 with 5% human blood, 7H10 with mycobactin-J,
       chocolate agar) was often unsuccessful in our laboratory but growth
       usually occurred by sub-culturing to 7H9 broth. Isolates from 4 of the
       patients have been identified by the California State Dept of Health as
       M. genavense. Identification of the isolates from the remaining patients
       is still in progress. The results of this study indicate that isolation
       of fastidious mycobacteria from our patient population is not uncommon
       and that mycobacterial blood cultures from patients with AIDS should be
       held for > 6 weeks since one-half of the fastidious isolates were
       recovered after 42 days of incubation.
 DE    AIDS-Related Opportunistic Infections/BLOOD/*MICROBIOLOGY  Human
       Mycobacterium/CLASSIFICATION/GROWTH & DEVELOPMENT/*ISOLATION &  PURIF
       Sputum/MICROBIOLOGY  Tuberculosis/BLOOD/COMPLICATIONS/*MICROBIOLOGY
       MEETING ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).