Document 0877
 DOCN  M9480877
 TI    Parenteral or intranasal immunization with HIV gp160 formulated with
       proteosomes and/or PA adjuvants enhances epitope-specific IgG or IgA.
 DT    9410
 AU    Kaminski R; Amselem S; Levi M; Loomis L; Kersey K; Vancott T; Friedman
       D; Smith G; Wahren B; Redfield R; et al; Walter Reed Army Institute of
       Research, Washington, DC.
 SO    Abstr Gen Meet Am Soc Microbiol. 1994;94:155 (abstract no. E-70). Unique
       Identifier : AIDSLINE ASM94/94313068
 AB    We have immunized mice i.m. or intranasally and rabbits i.m. with HIV
       gp160 (MicroGeneSys) formulated with alum or proprietary adjuvants (PA,
       Pharmos) and/or meningococcal outer membrane protein proteosomes. When
       administered i.m., proteosomes plus alum or PA formulations (with or
       without proteosomes) induced improved and broadened immune responses
       against several gp160 epitopes compared to the standard gp160
       alum-adjuvanted vaccine in both mice and rabbits. Moreover, either PA or
       proteosomes dramatically enhanced immunogenicity following intranasal
       immunization and proteosome formulation markedly boosted IgA responses.
       Data analyzed using a combination of ELISA, quantitative Western Blot
       and PEPscan techniques show that altering the presentation, formulation
       and route of administration of gp160 influences both quantitative and
       qualitative aspects of epitope specific IgG and IgA immune responses.
       These data are encouraging for the development of enhanced i.m. or
       intranasal sub-unit formulations of gp160 vaccines for HIV vaccine
       therapy.
 DE    Adjuvants, Immunologic  Administration, Intranasal  Alum Compounds
       Animal  Antigenic Determinants/ANALYSIS  AIDS Vaccines/*ADMINISTRATION &
       DOSAGE/IMMUNOLOGY  Comparative Study  Cysteine Proteinases  Drug
       Carriers  Gene Products, env/ADMINISTRATION & DOSAGE/*IMMUNOLOGY
       HIV/*IMMUNOLOGY  HIV Antibodies/ANALYSIS/BIOSYNTHESIS
       IgG/ANALYSIS/*BIOSYNTHESIS  IgM/ANALYSIS/*BIOSYNTHESIS  Injections,
       Intramuscular  Mice  Multienzyme Complexes  Protein
       Precursors/ADMINISTRATION & DOSAGE/*IMMUNOLOGY  Rabbits  MEETING
       ABSTRACT

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).