Document 0304
 DOCN  M9490304
 TI    Delta opioid receptor gene expression in lymphocytes.
 DT    9411
 AU    Chuang LF; Chuang TK; Killam KF Jr; Chuang AJ; Kung HF; Yu L; Chuang RY;
       Department of Medical Pharmacology and Toxicology, University of;
       California, Davis 95616.
 SO    Biochem Biophys Res Commun. 1994 Aug 15;202(3):1291-9. Unique Identifier
       : AIDSLINE MED/94338340
 AB    Previous studies have shown that morphine stimulates simian
       immunodeficiency virus (SIV) replication in SIV-infected human CEM x174
       cells as well as in monkey lymphocytes through a mechanism of delaying
       the lysis of infected cells (Biochem. Biophys. Res. Commun.
       195:1165-1173, 1993). The present study describes the identification of
       brain-like opioid receptor sequences in RNA transcripts of both CEM x174
       cells and monkey lymphocytes. Study of the gene sequence of a lymphocyte
       opioid receptor encompassing the third transmembrane domain and the
       third cytoplasmic loop indicates a 96% homology in amino acid
       composition to the delta opioid receptor in brain cells. Expression of
       such an opioid receptor sequence in lymphocytic cells is constitutive,
       since it could be detected in both saline-treated and morphine-treated
       monkeys as well as in morphine-treated monkeys after detoxification.
 DE    Amino Acid Sequence  Animal  Base Sequence  Blotting, Southern
       Brain/METABOLISM  Cells, Cultured  DNA Primers  *Gene Expression  Human
       Lymphocytes/*METABOLISM  Macaca mulatta  Male  Molecular Sequence Data
       Polymerase Chain Reaction  Receptors, Opioid, delta/*GENETICS  RNA,
       Messenger/GENETICS/METABOLISM  Sequence Homology, Amino Acid  Support,
       U.S. Gov't, P.H.S.  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).