Document 0324
 DOCN  M9490324
 TI    [The use of a primary astrocyte culture for modelling HIV neurological
       infection]
 DT    9411
 AU    Rytik PG; Kvacheva ZB; Eremin VF; Poleshchuk NN; Popov SA; Muller WB;
       Asher DM
 SO    Zh Mikrobiol Epidemiol Immunobiol. 1993 Mar-Apr;(2):47-52. Unique
       Identifier : AIDSLINE MED/94337564
 AB    The article deals with the results of the experiment substantiating the
       in vitro model of HIV neuro-infection. In this work primary glial
       (astrocytic) tissue cultures obtained from normal human and animal
       (guinea pig) brain tissue were used. As revealed in this investigation,
       the following phenomena could be observed in human brain tissue
       monolayer culture, infected with HIV and subsequently subcultured: (a)
       the stimulation of tissue-cell growth; (b) the formation of multinuclear
       glial cells; (c) the presence of virus-specific proteins in astrocyte
       cytoplasm, detected by immunofluorescent and electrophoretic techniques;
       (d) the presence of HIV-1 DNA provirus in infected astrocytes.
       Cyto-destruction was not observed, reverse transcriptase activity was
       absent.
 DE    Animal  Animals, Newborn  Astrocytes/*MICROBIOLOGY/ULTRASTRUCTURE  AIDS
       Dementia Complex/*MICROBIOLOGY/PATHOLOGY  Cell Line  Cells, Cultured
       *Disease Models, Animal  English Abstract  Guinea Pigs  Human  *HIV-1
       Microscopy, Electron  Microscopy, Phase-Contrast  JOURNAL ARTICLE

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