Document 0333
 DOCN  M9490333
 TI    [The isolation and characteristics of the green monkey lentivirus]
 DT    9411
 AU    Rezikian SA; Voevodin AF; Klots IN; Shtutman MS; Lapin BA
 SO    Vopr Virusol. 1993 Mar-Apr;38(2):76-8. Unique Identifier : AIDSLINE
       MED/94337516
 AB    We characterized the simian immunodeficiency virus isolated from
       Cercopithecus aethiops (subspecies C. a. pygerythrus) originating from
       Kenya. SIV was isolated and continuously produced with the MOLT4 clone 8
       cell line and was designated as SIV-SU1. SIV-SU1 isolate replicated with
       high efficiency in MOLT4 clone 8, MT-2 with moderate efficiency in CEM x
       174 and with poor efficiency in HUT-78, U937, C8166. The infection of
       MT-2, C8166 and HUT-78 resulted in extensive cell killing. Western
       blotting of purified preparations of SIV-SU1 revealed viral proteins of
       130, 68, 55, 41, 24, 17 kDa. Cross-reactivity of SIV-SU1 proteins with
       HIV-1, HIV-2, SIVmac, SIVsm, SIVmnd was studied by
       radioimmunoprecipitation assay. The most extensive cross-reactivity was
       observed with SIVmac. Total cellular DNA from chronically infected cells
       was hybridized to SIVagm266 DNA probes. Detection of cross-hybridizing
       DNA sequences required very low stringency, and the restriction
       endonuclease fragmentation pattern of SIV-SU1 differed from other SIVs.
 DE    Animal  Antigens, Viral/ANALYSIS  Cercopithecus aethiops/*MICROBIOLOGY
       Cross Reactions  DNA, Viral/GENETICS  Electrophoresis, Polyacrylamide
       Gel  English Abstract  Human  Immunoblotting  Lymphocytes/MICROBIOLOGY
       Radioimmunoassay  Restriction Mapping
       SIV/GENETICS/IMMUNOLOGY/*ISOLATION & PURIF/PHYSIOLOGY  Virus Cultivation
       Virus Replication  JOURNAL ARTICLE

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