Document 0369
 DOCN  M9490369
 TI    In situ detection of polymerase chain reaction-amplified HIV-1 nucleic
       acids in skeletal muscle in patients with myopathy.
 DT    9411
 AU    Seidman R; Peress NS; Nuovo GJ; Department of Pathology, SUNY, Stony
       Brook.
 SO    Mod Pathol. 1994 Apr;7(3):369-75. Unique Identifier : AIDSLINE
       MED/94336630
 AB    The purpose of this study was to determine if the myopathy that commonly
       occurs in patients with AIDS is associated with active HIV-1 infection
       in the muscle tissues. Seven muscle biopsies from patients infected by
       HIV-1 and six controls were tested for HIV-1 DNA and RNA using
       polymerase chain reaction in situ hybridization and reverse
       transcriptase in situ polymerase chain reaction. HIV-1 DNA was detected
       in rare cells in only one case by standard in situ hybridization.
       However, after polymerase chain reaction amplification HIV-1 DNA was
       detected in many cells in four of seven muscle tissues from patients
       with the viral infection and in none of the controls. The number of
       cells with detectable provirus in the tissue positive by standard in
       situ hybridization increased up to 100-fold after amplification. Most of
       the HIV-1 infected cells were macrophages, as determined by colabeling
       experiments that were localized mainly in the areas of myocyte necrosis.
       Myocyte nuclei that contained amplified HIV-1 nucleic acids were also
       noted. Most virally infected cells contained HIV-1 transcripts, which is
       consistent with activated infection. The demonstration of many HIV-1
       infected macrophages and myocytes in muscle biopsies from HIV-1 infected
       patients with myopathy suggests that active viral infection may play a
       role in the clinical disease state.
 DE    Adult  Aged  DNA, Viral/*ANALYSIS  Female  Human  HIV
       Infections/*MICROBIOLOGY  HIV-1/*GENETICS  Immunoenzyme Techniques  In
       Situ Hybridization  Male  Middle Age  Muscles/*MICROBIOLOGY/PATHOLOGY
       Myositis/*MICROBIOLOGY/PATHOLOGY  Polymerase Chain Reaction  RNA,
       Viral/*ANALYSIS  Support, Non-U.S. Gov't  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).