Document 0408
 DOCN  M9490408
 TI    Analysis of a rape case by direct sequencing of the human
       immunodeficiency virus type 1 pol and gag genes.
 DT    9411
 AU    Albert J; Wahlberg J; Leitner T; Escanilla D; Uhlen M; Department of
       Clinical Virology, Swedish Institute for Infectious; Disease Control,
       Karolinska Institute, Stockholm.
 SO    J Virol. 1994 Sep;68(9):5918-24. Unique Identifier : AIDSLINE
       MED/94335109
 AB    Transmission of human immunodeficiency virus type 1 (HIV-1) from a male
       accused of rape and deliberate transmission of HIV-1 was investigated by
       sequencing of the HIV-1 pol and gag genes from virus obtained from the
       male and from the female victim. Parts of the reverse transcriptase and
       p17gag genes were amplified and directly sequenced from uncultured
       peripheral blood mononuclear cells. The sequences were compared with
       sequences from 21 unrelated HIV-1-infected controls from the same
       geographic area (Stockholm, Sweden). Bootstrap analysis of phylogenetic
       trees demonstrated that the sequences from the female were significantly
       more closely related to the sequences from the male than to sequences
       from the controls. Furthermore, we found that the male and female shared
       two distinct genetic variants of HIV-1. In p17gag the major variant had
       an unusual, out-of-frame deletion of 3 nucleotides which the minor
       variant lacked. These results indicated that the male had transmitted
       more than one infectious unit to the female. From this study we
       concluded that it was highly likely that the HIV-1 strains carried by
       the male and female were closely epidemiologically linked.
 DE    Base Sequence  Comparative Study  Drug Resistance, Microbial  DNA
       Primers/CHEMISTRY  Female  *Genes, gag  *Genes, pol  Human
       HIV-1/*GENETICS  Male  Molecular Sequence Data  Phylogeny  *Rape
       Reverse Transcriptase/CHEMISTRY  Sequence Alignment  Sequence Homology,
       Amino Acid  Support, Non-U.S. Gov't  Zidovudine/PHARMACOLOGY  JOURNAL
       ARTICLE

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       protected by Copyright Law (Title 17, U.S.Code).