Document 0414
 DOCN  M9490414
 TI    Induction of feline immunodeficiency virus-specific cytotoxic T cells in
       vivo with carrier-free synthetic peptide.
 DT    9411
 AU    Flynn JN; Cannon CA; Beatty JA; Mackett M; Rigby MA; Neil JC; Jarrett C;
       Department of Veterinary Pathology, University of Glasgow,; Bearsden,
       United Kingdom.
 SO    J Virol. 1994 Sep;68(9):5835-44. Unique Identifier : AIDSLINE
       MED/94335100
 AB    The role of cellular immunity in the establishment and progression of
       immunosuppressive lentivirus infection remains equivocal. To develop a
       model system with which these aspects of the host immune response can be
       studied experimentally, we examined the response of cats to a hybrid
       peptide containing predicted T-and B-cell epitopes from the gag and env
       genes of feline immunodeficiency virus (FIV). Cats were immunized with
       an unmodified 17-residue peptide incorporating residues 196 to 208 (from
       gag capsid protein p24) and 395 to 398 (from env glycoprotein gp120) of
       the FIV Glasgow-8 strain by using Quil A as an adjuvant. Virus-specific
       lymphocytotoxicity was measured by chromium-51 release assays. The
       target cells were autologous or allogeneic skin fibroblasts either
       infected with recombinant FIV gag vaccinia virus or pulsed with FIV
       peptides. Effector cells were either fresh peripheral blood mononuclear
       cells or T-cell lines stimulated with FIV peptides in vitro. Cytotoxic
       effector cells from immunized cats lysed autologous, but not allogeneic,
       target cells when they were either infected with recombinant FIV gag
       vaccinia virus or pulsed with synthetic peptides comprising residues 196
       to 205 or 200 to 208 plus 395. Depletion of CD8+ T cells, from the
       effector cell population abrogated the lymphocytotoxicity. Immunized
       cats developed an antibody response to the 17-residue peptide immunogen
       and to recombinant p24. However, no antibodies which recognized smaller
       constituent peptides could be detected. This response correlated with
       peptide-induced T-cell proliferation in vitro. This study demonstrates
       that cytotoxic T lymphocytes specific for FIV can be induced following
       immunization with an unmodified short synthetic peptide and defines a
       system in which the protective or pathological role of such responses
       can be examined.
 DE    Amino Acid Sequence  Animal  Antibodies, Viral/IMMUNOLOGY  Antigens,
       CD8/ANALYSIS  Cats  Gene Products, gag/CHEMISTRY/*IMMUNOLOGY
       Immunodeficiency Virus, Feline/*IMMUNOLOGY  Lymphocyte Transformation
       Molecular Sequence Data  Peptides/*IMMUNOLOGY  Support, Non-U.S. Gov't
       T-Lymphocyte Subsets/IMMUNOLOGY  T-Lymphocytes, Cytotoxic/*IMMUNOLOGY
       Vaccines, Synthetic  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).