Document 0416
 DOCN  M9490416
 TI    The herpes simplex virus type 1 major capsid protein (VP5-UL19) promoter
       contains two cis-acting elements influencing late expression.
 DT    9411
 AU    Huang CJ; Wagner EK; Department of Molecular Biology and Biochemistry,
       University of; California, Irvine 92717.
 SO    J Virol. 1994 Sep;68(9):5738-47. Unique Identifier : AIDSLINE
       MED/94335089
 AB    The herpes simplex virus type 1 (HSV-1) major capsid protein VP5 gene
       (UL19) is expressed with beta gamma (gamma 1 [leaky late]) kinetics. We
       have previously described the construction of recombinant HSV-1 in which
       the VP5 promoter was engineered to control the expression of the
       bacterial beta-galactosidase gene as a reporter (C.-J. Huang, S. A.
       Goodart, M. K. Rice, J. F. Guzowski, and E. K. Wagner, J. Virol.
       67:5109-5116, 1993). Here we describe further mutational analysis in
       recombinant viruses. We have precisely defined the boundaries of the VP5
       promoter and identified two regions important for both the level and the
       kinetics of expression. The 5' boundary was located at -48 relative to
       the initiation site of transcription by analyzing a series of nested
       deletions in the upstream sequence, and although a number of cis-acting
       sites influencing transient expression have been identified upstream of
       this point, these sites have no role in promoter activity during
       productive infection. Deletion of an Sp1-binding site located between
       -48 and the TATA box at 30 greatly reduced VP5 promoter activity late
       but not early after infection. A cis-acting element whose sequence
       resembles the human immunodeficiency virus type 1 initiator was located
       between -2 and +10 in the VP5 sequence by characterizing a series of
       deletions and site-directed block mutations downstream the TATA box.
       This element defines the 3' limit of the VP5 promoter, and like the
       upstream element, disruption of this element also inhibited promoter
       activity late in the productive cycle.
 DE    Base Sequence  Binding Sites  Capsid/*GENETICS  Consensus Sequence  DNA
       Mutational Analysis  *Gene Expression Regulation, Viral  Herpesvirus 1,
       Human/*GENETICS  Molecular Sequence Data  Mutagenesis, Site-Directed
       Promoter Regions (Genetics)  RNA, Messenger/GENETICS  Sequence Deletion
       Support, U.S. Gov't, P.H.S.  Transcription Factor, Sp1/METABOLISM
       Transcription, Genetic  JOURNAL ARTICLE

       SOURCE: National Library of Medicine.  NOTICE: This material may be
       protected by Copyright Law (Title 17, U.S.Code).