Document 0419
 DOCN  M9490419
 TI    Detection and characterization of a functional complex of human
       immunodeficiency virus type 1 integrase and its DNA substrate by UV
       cross-linking.
 DT    9411
 AU    Yoshinaga T; Kimura-Ohtani Y; Fujiwara T; Shionogi Institute for Medical
       Science, Osaka, Japan.
 SO    J Virol. 1994 Sep;68(9):5690-7. Unique Identifier : AIDSLINE
       MED/94335083
 AB    To analyze the association of the human immunodeficiency virus type 1
       integrase (IN) protein with DNA substrates, we applied a shortwave UV
       cross-linking method to the in vitro integration system. Three
       photoadduct bands were detected on sodium dodecyl sulfate-polyacrylamide
       gels. The appearances of these photoadducts were examined with various
       substrates and under several incubation conditions. Our data suggest
       that one of these photoadducts is derived from the sequence- and
       strand-specific bound state of the early phase of the integration
       reaction before the 3' processing reaction. We also show that most of
       the photoadduct complexes are competent for integration even after UV
       irradiation.
 DE    Base Sequence  DNA Nucleotidyltransferases/*METABOLISM  DNA-Binding
       Proteins/METABOLISM  DNA, Viral/*METABOLISM  Ethylmaleimide/CHEMISTRY
       HIV-1/*ENZYMOLOGY  Molecular Sequence Data
       Oligodeoxyribonucleotides/CHEMISTRY  Photochemistry  Structure-Activity
       Relationship  Time Factors  Ultraviolet Rays  *Virus Integration
       JOURNAL ARTICLE

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